CAJ | 학술논문

目的研究miR-186靶向PIG11基因抑制胃癌细胞的侵袭。方法首先通过生物信息学方法分析，寻找与PIG11（TP53I11）结合的靶miRNAs。分别构建miR-186 mimics、点突变序列（Point mutation，PM）、空白载体（Scramble），转染胃癌MKN-28细胞。qRT-PCR检测miR-186表达，Western blot 检测PIG11表达，分析miR-186表达和PIG11表达之间的相关性。 Transwell检测转染前后各组胃癌MKN-28细胞侵袭能力变化。结果生物信息学发现12个miRNAs可能是PIG11的潜在靶miRNAs，其中miR-186与PIG11结合特异性最好、稳定性最强；MKN-28-miR-186 mimics细胞株中miR-186表达增高，而PIG11表达下调，而MKN-28、MKN-28-miR-Scramble、MKN-28-miR-186-PM 3个细胞株中miR-186表达水平较低，而PIG11表达水平较高。 MKN-28-miR-186 mimics侵袭细胞计数明显低于其他3组（P＜0.05）。结论 miR-186有可能靶向结合PIG11，下调其表达并抑制胃癌细胞的侵袭。
목적연구miR-186파향PIG11기인억제위암세포적침습。방법수선통과생물신식학방법분석，심조여PIG11（TP53I11）결합적파miRNAs。분별구건miR-186 mimics、점돌변서렬（Point mutation，PM）、공백재체（Scramble），전염위암MKN-28세포。qRT-PCR검측miR-186표체，Western blot 검측PIG11표체，분석miR-186표체화PIG11표체지간적상관성。 Transwell검측전염전후각조위암MKN-28세포침습능력변화。결과생물신식학발현12개miRNAs가능시PIG11적잠재파miRNAs，기중miR-186여PIG11결합특이성최호、은정성최강；MKN-28-miR-186 mimics세포주중miR-186표체증고，이PIG11표체하조，이MKN-28、MKN-28-miR-Scramble、MKN-28-miR-186-PM 3개세포주중miR-186표체수평교저，이PIG11표체수평교고。 MKN-28-miR-186 mimics침습세포계수명현저우기타3조（P＜0.05）。결론 miR-186유가능파향결합PIG11，하조기표체병억제위암세포적침습。
Objective To study the mechanism of miR-186 targeted to PIG11 and inhibit metastasis and invasion of gastric cancer cells. Methods Bioinformatics were used to analyze the miRNAs targeted to PIG11. qRT-PCR detected the level of miR-186 in gastric cancer cell lines MKN-28. The expression of PIG11 were detected by Western blot. And the changes of ability of metastasis and invasion were examined by Transwell. Results There were high expression of miR-186 and low expression of PIG11 in MKN-28-miR-186 mimics. There were low expression of miR-186 and high expression of PIG11 in MKN-28, MKN-28-miR-Scramble, MKN-28-miR-186-PM(P<0.05). There were smaller invaded cells in MKN-28-miR-186 mimics than in other three cell lines (P<0.05). Conclusion miR-186 can target to PIG11 and down regulate its expression and inhibit metastasis and invasion of gastric cancer cells.