CAJ | 학술논문

目的：探讨溃疡性结肠炎（UC）患者大肠黏膜组织中凋亡蛋白半乳糖凝集素3（Gal‐3）与B淋巴细胞瘤‐2基因（Bcl‐2）的表达及意义。方法应用免疫组织化学SP法检测UC患者60例（UC组）和正常对照者20例（对照组）大肠黏膜组织中的Gal‐3与Bcl‐2的表达，并分析UC大肠黏膜组织中Gal‐3与Bcl‐2表达的关系。以细胞质或细胞核中出现明显的棕黄色颗粒为阳性细胞，根据染色强度及阳性细胞数进行光镜下半定量分析。结果Gal‐3与Bcl‐2的表达均在上皮细胞的细胞质，Gal‐3在正常大肠黏膜上皮细胞中呈强阳性表达，在UC大肠上皮细胞的表达明显减弱，且与肠镜下病变程度相关；Bcl‐2在正常大肠黏膜上皮细胞中呈弱表达，在UC大肠黏膜上皮细胞的表达明显增强，主要为固有层炎性细胞表达增强，与肠镜下病变程度无明显关系。Gal‐3与Bcl‐2的表达与患者的年龄、性别、病程均无明显相关。Pearson相关分析显示Gal‐3与Bcl‐2的阳性表达程度不具有关联性。结论Gal‐3与Bcl‐2蛋白在UC的发生和发展中起重要作用，可望成为UC早期诊断和预后判断的指标，两者在UC的发病中可能独立起作用。
목적：탐토궤양성결장염（UC）환자대장점막조직중조망단백반유당응집소3（Gal‐3）여B림파세포류‐2기인（Bcl‐2）적표체급의의。방법응용면역조직화학SP법검측UC환자60례（UC조）화정상대조자20례（대조조）대장점막조직중적Gal‐3여Bcl‐2적표체，병분석UC대장점막조직중Gal‐3여Bcl‐2표체적관계。이세포질혹세포핵중출현명현적종황색과립위양성세포，근거염색강도급양성세포수진행광경하반정량분석。결과Gal‐3여Bcl‐2적표체균재상피세포적세포질，Gal‐3재정상대장점막상피세포중정강양성표체，재UC대장상피세포적표체명현감약，차여장경하병변정도상관；Bcl‐2재정상대장점막상피세포중정약표체，재UC대장점막상피세포적표체명현증강，주요위고유층염성세포표체증강，여장경하병변정도무명현관계。Gal‐3여Bcl‐2적표체여환자적년령、성별、병정균무명현상관。Pearson상관분석현시Gal‐3여Bcl‐2적양성표체정도불구유관련성。결론Gal‐3여Bcl‐2단백재UC적발생화발전중기중요작용，가망성위UC조기진단화예후판단적지표，량자재UC적발병중가능독립기작용。
Objective To explore the expressions and significance of Galectin‐3 and Bcl‐2 in colorectal tissues of patients with ulcerative colitis(UC) .Methods Immunohistochemical SP method was applied to detected the expression of Galectin‐3 and Bcl‐2 in colorectal tissues of 60 patients in UC group and 20 healthy adults in the control group ,and analyzed the relationship of the expres‐sions between Galectin‐3 and Bcl‐2 .It was regarded as positive cell when obvious dark brown granules appeared in cytoplasm or cyteblast .Semi‐quantitative analysis was used basing on the staining intensity and the amount of the staining intensity and positive cells .Results Galectin‐3 and Bcl‐2 proteins expressed in cytoplasm .Galectin‐3 showed strong expression in normal colorectal epi‐thelium but weak in UC inflammatory tissues ,and it was associated with different lesion degrees under endoscopy .The expressions of Bcl‐2 were weak in normal colorectal epithelium ,and it enhanced significantly in UC inflammatory tissues ,especially in inflamma‐tory cells of laminae propria ,and it was not associated with different lesion degrees under endoscopy .The expression of Galectin‐3 and Bcl‐2 was not associated with the age ,sex of patients and the course of UC .Pearson correlation analysis showed that the posi‐tive expressions of Galectin‐3 and Bcl‐2 had no relevance .Conclusion Galectin‐3 and Bcl‐2 involved in the pathogenesis of UC . They may be able to used as markers of early diagnosis and prognosis in UC and may play the role in the pathogenesis of UC inde‐pendently .