CAJ | 학술논문

目的：观察秦皮素对苯肾上腺素诱导的原代SD乳鼠心肌细胞肥大的影响。方法建立苯肾上腺素诱导的SD乳鼠原代心肌细胞肥大模型，观察秦皮素对心肌细胞肥大的影响；图像分析法计算心肌细胞面积；[3H]‐亮氨酸掺入法测试心肌细胞蛋白质合成速率；实时定量荧光PCR法检测心肌细胞Nrf2和肥大分子标志物心房钠尿肽、心房利尿肽的mRNA表达水平。结果（1）80μmol／L苯肾上腺素刺激心肌细胞48h能成功复制SD乳鼠心肌细胞肥大模型并伴随Nrf2表达量的增加；（2）秦皮素呈剂量依赖性地抑制苯肾上腺素刺激的SD乳鼠心肌细胞面积的增大、蛋白质合成速率的增加和肥大分子标志物的上调。结论秦皮素能显著抑制PE 诱导的原代大鼠心肌细胞肥大。
목적：관찰진피소대분신상선소유도적원대SD유서심기세포비대적영향。방법건립분신상선소유도적SD유서원대심기세포비대모형，관찰진피소대심기세포비대적영향；도상분석법계산심기세포면적；[3H]‐량안산참입법측시심기세포단백질합성속솔；실시정량형광PCR법검측심기세포Nrf2화비대분자표지물심방납뇨태、심방이뇨태적mRNA표체수평。결과（1）80μmol／L분신상선소자격심기세포48h능성공복제SD유서심기세포비대모형병반수Nrf2표체량적증가；（2）진피소정제량의뢰성지억제분신상선소자격적SD유서심기세포면적적증대、단백질합성속솔적증가화비대분자표지물적상조。결론진피소능현저억제PE 유도적원대대서심기세포비대。
Objective To investigate the effect of fraxetin on primary cardiomyocyte hypertrophy induced by phenylephrine . Methods Primary SD cardiomyocyte hypertrophy was induced by phenylephrine ,and we observed the effects of fraxetin on cardio‐myocyte hypertrophy induced by phenylephrine .Image‐ProPlus 5 measured the area of cardiomyocyte;[3 H ]‐leucine incorporation assay detected the protein synthesis rate of cardiomyocyte;Real‐time PCR measured the Nrf2 and molecular markers (ANP ,BNP) mRNA expression levels of cardiomyyocyte hypertrophy .Results (1)Primary neonate SD Cardiomyocyte hypertrophy model was successfully established by 80 μmol/L phenylephrine for 48 h ,and Nrf2 expression levels significantly increased in cardiomyocyte hypertrophy model;(2)The increase of cardiomyocyte area ,protein synthesis rate and molecular markers expression of cardiomyyo‐cyte hypertrophy were significantly inhibited by fraxetin in a dose‐dependent manner .Conclusion Fraxetin could significantly inhib‐it the cardiomyyocyte hypertrophy induced by PE .