CAJ | 학술논문

目的：探讨E‐钙粘蛋白（E‐cad）在急性胰腺炎中的表达及对其严重性的诊断价值。方法24只雄性SD大鼠随机均分为对照组、轻症急性胰腺炎组（MAP组）和重症急性胰腺炎组（SAP组）。通过腹腔注射L‐精氨酸造模，建模24h后测定血清中淀粉酶活性及E‐cad表达变化，胰腺组织进行病理组织学观察，蛋白免疫印迹（Westernblot）检测胰腺组织E‐cad的蛋白表达。结果MAP组和SAP组胰腺病理评分、血清淀粉酶值明显高于对照组（P＜0．05），SAP组与MAP组比较差异也有统计学意义（P＜0．05）；血清E‐cad酶联免疫吸附试验（ELISA）检测结果显示：MAP组（1025．50±131．33）pg／mL和SAP组（1561．75±144．82）pg／mL均明显高于对照组（626．50±72．12）pg／mL，且两组之间比较差异也有统计学意义（P＜0．05）；Westernblot检测胰腺组织中SAP组E‐cad表达强于对照组及MAP组。结论E‐cad在急性胰腺炎早期表达明显增高，对其严重性具有一定的诊断价值。
목적：탐토E‐개점단백（E‐cad）재급성이선염중적표체급대기엄중성적진단개치。방법24지웅성SD대서수궤균분위대조조、경증급성이선염조（MAP조）화중증급성이선염조（SAP조）。통과복강주사L‐정안산조모，건모24h후측정혈청중정분매활성급E‐cad표체변화，이선조직진행병리조직학관찰，단백면역인적（Westernblot）검측이선조직E‐cad적단백표체。결과MAP조화SAP조이선병리평분、혈청정분매치명현고우대조조（P＜0．05），SAP조여MAP조비교차이야유통계학의의（P＜0．05）；혈청E‐cad매련면역흡부시험（ELISA）검측결과현시：MAP조（1025．50±131．33）pg／mL화SAP조（1561．75±144．82）pg／mL균명현고우대조조（626．50±72．12）pg／mL，차량조지간비교차이야유통계학의의（P＜0．05）；Westernblot검측이선조직중SAP조E‐cad표체강우대조조급MAP조。결론E‐cad재급성이선염조기표체명현증고，대기엄중성구유일정적진단개치。
Objective To explore the diagnosis value and expression of E‐cadherin in the acute pancreatitis .Methods Twenth‐four male SD rats were randomly divided into control group ,mild acute pancreatitis (MAP) group and severe acute pancreatitis (SAP) group ,8 case in each groups .The animal model was established by intraperitoneal injection of L‐arginine .After 24 hours ,the serum levels of amylase and the expression change of variation E‐cadherin were tested .The pancreas tissue was taken for his‐topathological score under microscope .The expression of E‐cadherin at protein level in pancreatic tissue was detected by Western Blot .Results The histological scores of pancreas and serum amylase were significantly higher in the MAP and SAP groups than those in control group (P<0 .05) ,and difference between SAP group and MAP group was also very significant(P<0 .05) .The re‐sult of ELISA showed that the serum E‐cadherin in MAP group[(1 025 .50 ± 131 .33) pg/mL] and SAP group[(1 561 .75 ± 144 .82)pg/mL] were significantly higher than that in control group[(626 .50 ± 72 .12) pg/mL ,P<0 .05] ,and there was significant difference between MAP group and SAP group(P<0 .05) .The result of Western Blot was similar to the result of ELISA .Conclu‐sion E‐cadherin may have diagnosis value in the acute pancreatitis .