重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2015年
2期
165-167,170
,共4页
生物源单胺类%记忆%壮精合剂%初老大鼠
生物源單胺類%記憶%壯精閤劑%初老大鼠
생물원단알류%기억%장정합제%초로대서
biogenic monoamines%memory%Zhuang Jing mixture(ZJM),primary senile rat
目的：观察壮精合剂对初老大鼠行为学及脑单胺类神经递质的影响，探讨其抗衰老的作用机制。方法选用12月龄，体质量（300±20）g。雌性初老大鼠50只，建立模型，分为5组：空白对照组、壮精合剂高剂量组、壮精合剂中剂量组、壮精合剂组低剂量组、阳性对照组，每组10只。各组分别灌胃相应药物8周后，采用Morris水迷宫检测各组大鼠的空间学习记忆行为能力及采用高效液相色谱‐荧光检测法检测各组大鼠大脑皮层、海马单胺类神经递质含量。结果与空白对照组比较，壮精合剂高、中剂量组及阳性对照组均能使初老大鼠学习记忆能力有所改善，并能提高其大脑皮层及海马区单胺类经递质含量。壮精合剂高剂量组与阳性对照组比较在改善学习记忆能力方面差异有统计学意义（P＜0．05），在提高大脑皮层及海马区单胺类经递质含量方面效果最为显著（P＜0．05）。结论壮精合剂能有效改善初老大鼠的学习记忆能力，其作用机制可能与调节大脑内单胺类神经递质有关。
目的：觀察壯精閤劑對初老大鼠行為學及腦單胺類神經遞質的影響，探討其抗衰老的作用機製。方法選用12月齡，體質量（300±20）g。雌性初老大鼠50隻，建立模型，分為5組：空白對照組、壯精閤劑高劑量組、壯精閤劑中劑量組、壯精閤劑組低劑量組、暘性對照組，每組10隻。各組分彆灌胃相應藥物8週後，採用Morris水迷宮檢測各組大鼠的空間學習記憶行為能力及採用高效液相色譜‐熒光檢測法檢測各組大鼠大腦皮層、海馬單胺類神經遞質含量。結果與空白對照組比較，壯精閤劑高、中劑量組及暘性對照組均能使初老大鼠學習記憶能力有所改善，併能提高其大腦皮層及海馬區單胺類經遞質含量。壯精閤劑高劑量組與暘性對照組比較在改善學習記憶能力方麵差異有統計學意義（P＜0．05），在提高大腦皮層及海馬區單胺類經遞質含量方麵效果最為顯著（P＜0．05）。結論壯精閤劑能有效改善初老大鼠的學習記憶能力，其作用機製可能與調節大腦內單胺類神經遞質有關。
목적：관찰장정합제대초로대서행위학급뇌단알류신경체질적영향，탐토기항쇠로적작용궤제。방법선용12월령，체질량（300±20）g。자성초로대서50지，건립모형，분위5조：공백대조조、장정합제고제량조、장정합제중제량조、장정합제조저제량조、양성대조조，매조10지。각조분별관위상응약물8주후，채용Morris수미궁검측각조대서적공간학습기억행위능력급채용고효액상색보‐형광검측법검측각조대서대뇌피층、해마단알류신경체질함량。결과여공백대조조비교，장정합제고、중제량조급양성대조조균능사초로대서학습기억능력유소개선，병능제고기대뇌피층급해마구단알류경체질함량。장정합제고제량조여양성대조조비교재개선학습기억능력방면차이유통계학의의（P＜0．05），재제고대뇌피층급해마구단알류경체질함량방면효과최위현저（P＜0．05）。결론장정합제능유효개선초로대서적학습기억능력，기작용궤제가능여조절대뇌내단알류신경체질유관。
Objective To observe the effect of Zhuang Jing mixture (ZJM ) on behavior of primary senile rat and monoamine neurotransmitters in it′s brain ,and to study its mechanism of anti‐aging .Methods Fifty senile female rates which average weight was(300 ± 20)g were randomized divided into 5 groups :blank control group ,low‐,mid‐and high‐dose group of ZJM ,positive control group ,with 10 rats in each group .After medication for 8 weeks ,Morris water maze test was used to evaluate the spatial learning and memory ability of primary senile rats .High performance liquid chromatography with fluorescence detection (HPLC‐FD) was used to detect the monoamine neurotransmitter content in rat′s cerebral cortex and hippocampus .Results Compared with blank control group ,high‐,middle‐dose group of ZJM and positive control group were improved the ability of learning and memory of pri‐mary senile rat ,as well as the cerebral cortex and hippocampus monoamine neurotransmitters levels .High‐dose group of ZJM had significant difference compared with positive control group in improving the ability of learning and memory(P<0 .05) ,and in im‐proving the cerebral cortex and hippocampus monoamine neurotransmitters(P<0 .05) .Conclusion ZJM could significantly improve the learning and memory abilities of primary senile rats ,and its mechanism may be related to adjust the monoamine neurotransmitter in brain .
