CAJ | 학술논문

目的：建立人结肠癌鸡胚移植模型，研究龙葵碱对其血管生成的影响。方法将鸡胚分为对照组和低剂量组、中剂量组、高剂量组（均n＝10），将培养的人结肠癌细胞系HT‐29细胞株接种到鸡胚绒毛尿囊膜（CAM）上，通过立体显微镜、Image‐proplus6．0图像分析软件及免疫组织化学苏木精‐伊红（HE）染色法，观察移植瘤在CAM上血管生成的特点，及不同龙葵碱剂量对血管生成的影响。结果HT‐29细胞接种到CAM第3～5天，大量血管向瘤体集中，长入或跨越瘤体表面，肿瘤迅速生长。给药后第5天进行拍照，图像分析，定量计算血管新生面积明显低于对照组，且呈剂量依赖性，各组间差异有统计学意义（P＜0．01）。免疫组织化学检测表明不同剂量龙葵碱的微血管密度明显低于对照组，与血管新生面积相一致；ki‐67抗原表达指数逐渐下降，实验组低于对照组，且各组间差异有统计学意义（P＜0．01）。结论龙葵碱能明显抑制人结肠癌HT‐29细胞株诱导的血管生成，从而抑制肿瘤的生长，为抗肿瘤血管生成的治疗方面提供了重要依据。
목적：건립인결장암계배이식모형，연구룡규감대기혈관생성적영향。방법장계배분위대조조화저제량조、중제량조、고제량조（균n＝10），장배양적인결장암세포계HT‐29세포주접충도계배융모뇨낭막（CAM）상，통과입체현미경、Image‐proplus6．0도상분석연건급면역조직화학소목정‐이홍（HE）염색법，관찰이식류재CAM상혈관생성적특점，급불동룡규감제량대혈관생성적영향。결과HT‐29세포접충도CAM제3～5천，대량혈관향류체집중，장입혹과월류체표면，종류신속생장。급약후제5천진행박조，도상분석，정량계산혈관신생면적명현저우대조조，차정제량의뢰성，각조간차이유통계학의의（P＜0．01）。면역조직화학검측표명불동제량룡규감적미혈관밀도명현저우대조조，여혈관신생면적상일치；ki‐67항원표체지수축점하강，실험조저우대조조，차각조간차이유통계학의의（P＜0．01）。결론룡규감능명현억제인결장암HT‐29세포주유도적혈관생성，종이억제종류적생장，위항종류혈관생성적치료방면제공료중요의거。
Objective To establish chicken embryo transplantation model of human colon cancer and to research the effect of so‐lanine on angiogenesis .Methods Cases with chicken embryos were divided into the low‐,mid‐and high dose solanine group and con‐trol group ,with 10 cases in each groups ,and then the cultured human colon cancer cell line HT‐29 cell lines were inoculated to the chicken embryo villus allantois membrane (CAM ) .We observed the characteristics of the transplanted tumor in CAM angiogenesis by the stereo microscope .Image analysis software of Image‐pro plus 6 .0 and immunohistochemical method were used to observe the effect of different dose of solanine on angiogenesis .Results HT‐29 cell lines were inoculated to CAM 3-5 days ,a large number of blood vessels concentrated in tumors ,growing into or acrossing the surface of tumors .While tumors also rapidly growed .We took photo on the 5th day after receiving medicine and did imaging analysis .Then we calculated the area of angiogenesis in experimental group ,which was significantly lower than that of the control group ,quantitatively in a dose‐dependent manner .There were signifi‐cant differences among the groups(P<0 .01) .Microvascular density of 3 different dose of solanine was significantly lower than that of the control group by immunohistochemical method ;the expression of Ki‐67 antigen index decreased gradually ,which was highest in the control group ,and there were significant differences among the groups (P<0 .01) .Conclusion Solanine could inhibit angio‐genesis induced by human colon cancer HT‐29 cell lines obviously ,thus inhibiting the growth of tumor and providing an important basis for the treatment of anti‐tumor angiogenesis .