CAJ | 학술논문

目的：观察抑郁复合皮肤创伤对创面新生微血管及成纤维细胞消长的影响。方法根据建模方法将144只SD大鼠随机分为单创组、创伤复合抑郁组、抑郁复合创伤组，每组48只，制备抑郁模型。分别采用CD34、Vimentin抗体行免疫组织化学标记血管内皮细胞及成纤维细胞，高倍显微镜下计数。结果伤后第3天单创组和创伤复合抑郁组大鼠的创面微血管数显著高于抑郁复合创伤组（P＜0．05），第21天时各组间微血管计数无显著差异；伤后第3天抑郁复合创伤组创面成纤维细胞虽少于单创组和创伤复合抑郁组，但差异无统计学意义（P＞0．05）；创伤后第18～21天，3组间成纤维细胞数量比较，差异有统计学意义（P＜0．05）。结论抑郁复合皮肤创伤时，创面肉芽组织中微血管及成纤维细胞的生长合成可被显著抑制，使伤口愈合延缓。
목적：관찰억욱복합피부창상대창면신생미혈관급성섬유세포소장적영향。방법근거건모방법장144지SD대서수궤분위단창조、창상복합억욱조、억욱복합창상조，매조48지，제비억욱모형。분별채용CD34、Vimentin항체행면역조직화학표기혈관내피세포급성섬유세포，고배현미경하계수。결과상후제3천단창조화창상복합억욱조대서적창면미혈관수현저고우억욱복합창상조（P＜0．05），제21천시각조간미혈관계수무현저차이；상후제3천억욱복합창상조창면성섬유세포수소우단창조화창상복합억욱조，단차이무통계학의의（P＞0．05）；창상후제18～21천，3조간성섬유세포수량비교，차이유통계학의의（P＜0．05）。결론억욱복합피부창상시，창면육아조직중미혈관급성섬유세포적생장합성가피현저억제，사상구유합연완。
Objective To explore the effect of depression combined with skin injury on wound capillary angiogenesis and fibro‐blast growth .Methods Totally 144 Sprague‐Dawley rats were randomly and equally divided into 3 groups:simple wound group ,de‐pression combined wound group ,wound combined depression group ,48 mice in each of the 3 groups .Immunohistochemical staining was used to label vascular endothelial cell and fibroblast with CD34 and Vimentin antibody respectively ,then counted by the high resolution microscope .Results Compared with other 2 groups ,the number of micro vascular and fibroblast in the wound granula‐tion tissue were lower in depression combined with mechanical skin injury group at the first stage(3 days after injury)(P<0 .05) , and faded more slowly in the later stage(at the 21st day)(P>0 .05) .There was significant difference between 3 groups in the num‐ber of fibroblast during the 18th day and 21st day(P<0 .05) .Conclusion Depression combined with skin injury could significantly inhibit the capillary angiogenesis and fibroblast growth in the wound granulation tissue ,thereby delaying wound healing .