北京医学
北京醫學
북경의학
BEIJING MEDICAL JOURNAL
2015年
1期
37-40,107
,共5页
徐舒敏%谢尹晶%郭宇妮%陈文燕%李芮冰%王正冠%王成彬
徐舒敏%謝尹晶%郭宇妮%陳文燕%李芮冰%王正冠%王成彬
서서민%사윤정%곽우니%진문연%리예빙%왕정관%왕성빈
感染%巨噬细胞炎性蛋白2%巨噬细胞炎性蛋白1α%白细胞介素6
感染%巨噬細胞炎性蛋白2%巨噬細胞炎性蛋白1α%白細胞介素6
감염%거서세포염성단백2%거서세포염성단백1α%백세포개소6
Infection%Macrophage inflammatory protein-2 (MIP-2)%Macrophage inflammatory protein-1α(MIP-1α)%Interleukin-6
目的：探讨巨噬细胞炎性蛋白2（macrophage inflammatory protein-2, MIP-2）、巨噬细胞炎性蛋白1α（macrophage inflammatory protein-1 Alpha, MIP-1α）和白细胞介素6（interleukin-6, IL-6）在金黄色葡萄球菌和大肠埃希菌感染时的变化规律及在鉴别诊断金黄色葡萄球菌和大肠埃希菌感染的价值。方法将216只ICR小鼠随机分为3组，各组72只。尾静脉注射大肠埃希菌和金黄色葡萄球菌建立小鼠血流感染模型，生理盐水注射建立阴性对照。共9个时间点，各时间点每组8只小鼠。分别在感染0.5h、1h、3h、6h、12h、1d、2d、3d、5d后采血，检测血液白细胞计数（WBC），血清中MIP-2、MIP-1α和IL-6浓度。结果①大肠埃希菌组和金黄色葡萄球菌组小鼠在感染0.5 h 后MIP-2、MIP-1α和IL-6均明显升高，各指标峰值分别为（13246.8±865.37）pg/ml 、（699.79±36.36）pg/ml、（25080.32±1263.58）pg/ml、（3483.22±169.84）pg/ml和（1171.98±104.49）pg/ml 、（94.71±14.38）pg/ml，大肠埃希菌感染组显著高于金黄色葡萄球菌感染组；②MIP-2、MIP-1α和IL-6在大肠埃希菌感染时的ROC曲线下面积（0.962、0.967、0.962）与金黄色葡萄球菌感染时的（0.877、0.889、0.885）的差异有统计学意义（P<0.05），并且两组之间差异也有统计学意义（P<0.05）。结论 MIP-2、MIP-1α和IL-6在炎症早期明显升高，在鉴别大肠埃希菌和金黄色葡萄球菌引发的感染均具有统计学意义。
目的：探討巨噬細胞炎性蛋白2（macrophage inflammatory protein-2, MIP-2）、巨噬細胞炎性蛋白1α（macrophage inflammatory protein-1 Alpha, MIP-1α）和白細胞介素6（interleukin-6, IL-6）在金黃色葡萄毬菌和大腸埃希菌感染時的變化規律及在鑒彆診斷金黃色葡萄毬菌和大腸埃希菌感染的價值。方法將216隻ICR小鼠隨機分為3組，各組72隻。尾靜脈註射大腸埃希菌和金黃色葡萄毬菌建立小鼠血流感染模型，生理鹽水註射建立陰性對照。共9箇時間點，各時間點每組8隻小鼠。分彆在感染0.5h、1h、3h、6h、12h、1d、2d、3d、5d後採血，檢測血液白細胞計數（WBC），血清中MIP-2、MIP-1α和IL-6濃度。結果①大腸埃希菌組和金黃色葡萄毬菌組小鼠在感染0.5 h 後MIP-2、MIP-1α和IL-6均明顯升高，各指標峰值分彆為（13246.8±865.37）pg/ml 、（699.79±36.36）pg/ml、（25080.32±1263.58）pg/ml、（3483.22±169.84）pg/ml和（1171.98±104.49）pg/ml 、（94.71±14.38）pg/ml，大腸埃希菌感染組顯著高于金黃色葡萄毬菌感染組；②MIP-2、MIP-1α和IL-6在大腸埃希菌感染時的ROC麯線下麵積（0.962、0.967、0.962）與金黃色葡萄毬菌感染時的（0.877、0.889、0.885）的差異有統計學意義（P<0.05），併且兩組之間差異也有統計學意義（P<0.05）。結論 MIP-2、MIP-1α和IL-6在炎癥早期明顯升高，在鑒彆大腸埃希菌和金黃色葡萄毬菌引髮的感染均具有統計學意義。
목적：탐토거서세포염성단백2（macrophage inflammatory protein-2, MIP-2）、거서세포염성단백1α（macrophage inflammatory protein-1 Alpha, MIP-1α）화백세포개소6（interleukin-6, IL-6）재금황색포도구균화대장애희균감염시적변화규률급재감별진단금황색포도구균화대장애희균감염적개치。방법장216지ICR소서수궤분위3조，각조72지。미정맥주사대장애희균화금황색포도구균건립소서혈류감염모형，생리염수주사건립음성대조。공9개시간점，각시간점매조8지소서。분별재감염0.5h、1h、3h、6h、12h、1d、2d、3d、5d후채혈，검측혈액백세포계수（WBC），혈청중MIP-2、MIP-1α화IL-6농도。결과①대장애희균조화금황색포도구균조소서재감염0.5 h 후MIP-2、MIP-1α화IL-6균명현승고，각지표봉치분별위（13246.8±865.37）pg/ml 、（699.79±36.36）pg/ml、（25080.32±1263.58）pg/ml、（3483.22±169.84）pg/ml화（1171.98±104.49）pg/ml 、（94.71±14.38）pg/ml，대장애희균감염조현저고우금황색포도구균감염조；②MIP-2、MIP-1α화IL-6재대장애희균감염시적ROC곡선하면적（0.962、0.967、0.962）여금황색포도구균감염시적（0.877、0.889、0.885）적차이유통계학의의（P<0.05），병차량조지간차이야유통계학의의（P<0.05）。결론 MIP-2、MIP-1α화IL-6재염증조기명현승고，재감별대장애희균화금황색포도구균인발적감염균구유통계학의의。
Objective To explore the variation of Macrophage Inflammatory Protein-2, Macrophage Inflammatory Protein-1Alpha ,interleukin-6 in mice with blood infection and analyze the correlation between them. The value of MIP-2, MIP-1α and IL-6 in differential diagnosis of Staphylococcus aureus infection and Escherichia coli infection were evaluat-ed. Methods 216 mices were divided into three groups randomly. Models of mices with bloodstream infection were estab-lished by being challenged with Escherichia coli and Staphylococcus aureus by intravenous tail injection. Seventy-two mice were challenged with isotonic sodium chloride solution as controls. WBC, MIP-2, MIP-1α and IL-6 were determined at 0.5 h,1 h, 3 h, 6 h, 12 h, 1 d, 2 d, 3 d and 5d after infections (n=8 per group). Results (1) MIP-2, MIP-1αand IL-6 all increased significantly at 0.5 h after infections and reached the peak at 6 h, (13 246.8±865.37)pg/ml, (595.4±28.3)pg/ml, (25 080.32±1 263.58)pg/ml, (2 509.13±272.05)pg/ml, (1 171.98±104.49)pg/ml, (94.71±14.38)pg/ml respectively. (2) The ar-eas under the ROC curve (AUC) of MIP-2, MIP-1α and IL-6 in two infecting groups were significantly different (P<0.05). Conclusion MIP-2, MIP-1α and IL-6 increase significantly early. All of them can be used to differentiate Es-cherichia coli and Staphylococcus aureus infections.
