CAJ | 학술논문

目的：研究Fas配体（FasL）及FasL反义寡脱氧核苷酸（ASODN）在舌鳞癌细胞免疫逃逸中的作用。方法对人舌鳞癌细胞中FasL表达及功能进行检测，设计合成特异FasLASODN，转染舌鳞癌细胞，封闭舌鳞癌细胞的FasL表达。通过流式细胞术、逆转录聚合酶链反应（RT‐PCR）和四唑盐（MTT）等方法，观察基因‐蛋白‐细胞效应。结果通过RT‐PCR检测提示在脂质体的量一定的情况下，在一定范围内，ASO的浓度越高，转染效率越高。MTT／FCM结果显示ASO对SCC‐9细胞的增殖有明显的抑制作用（P＜0．05）。FCM对细胞凋亡的检测结果显示反义链组与正义链组、空白对照组及脂质体组相比，总凋亡率有明显差异（P＜0．05）。结论Fas／FasL途径是口腔鳞癌细胞免疫逃逸的重要机制之一，可作为免疫治疗的新靶点。
목적：연구Fas배체（FasL）급FasL반의과탈양핵감산（ASODN）재설린암세포면역도일중적작용。방법대인설린암세포중FasL표체급공능진행검측，설계합성특이FasLASODN，전염설린암세포，봉폐설린암세포적FasL표체。통과류식세포술、역전록취합매련반응（RT‐PCR）화사서염（MTT）등방법，관찰기인‐단백‐세포효응。결과통과RT‐PCR검측제시재지질체적량일정적정황하，재일정범위내，ASO적농도월고，전염효솔월고。MTT／FCM결과현시ASO대SCC‐9세포적증식유명현적억제작용（P＜0．05）。FCM대세포조망적검측결과현시반의련조여정의련조、공백대조조급지질체조상비，총조망솔유명현차이（P＜0．05）。결론Fas／FasL도경시구강린암세포면역도일적중요궤제지일，가작위면역치료적신파점。
Objective To research the effects of FasL and FasL ASODN in the immune escape of squamous lingual carcinoma cells .Methods This experiment was planned to conduct the test through the expression and functions of FasL in squamous lingual carcinoma cells ,and designed and compounded the specific FasL ASODN to transfect squamous lingual carcinoma cells and block the expression of FasL in squamous lingual carcinoma cells .Such methods as RT‐PCR ,flow cytometry and M T T had been adopted to conduct the observation from three levels ,gene ,protein and cell effects .Results The FasL ASO inhibited cell proliferation in a dose‐and time‐dependent manner within limits(P<0 .05) .The total apoptosis rate was apparently increased(P<0 .05) in antisense group compared with the control group ,the lipofectamine group and the sense group .Conclusion Fas/FasL approach is one of the important mechanisms of the immune escape of oral squamous cell carcinoma cells ,and can be the new target for immunotherapy .