CAJ | 학술논문

目的：研究人21．5kDa人脑髓鞘碱性蛋白（MBP）基因沉默对神经胶质瘤U251细胞增殖与凋亡的影响。方法将21．5kDaMBP序列特异性短发夹RNA（shRNA）重组质粒pGenesil‐1‐MBP‐3转染神经胶质瘤细胞株（U251）作为MBP基因沉默组，以空质粒转染为阴性对照组，脂质体转染为脂质体空转组；用实时定量PCR（RT‐PCR）和Westernblot方法检测各组21．5kDaMBPmRNA和蛋白的表达水平，CCK‐8法测定细胞增殖曲线，流式细胞法分析细胞凋亡率。结果与阴性对照组相比，MBP基因沉默组细胞中21．5kDaMBP在mRNA和蛋白水平的表达均显著下降（P＜0．05）、细胞增殖活性明显降低（P＜0．05）、细胞凋亡率显著增高（P＜0．05）。结论人21．5kDaMBP基因沉默对神经胶质瘤细胞U251具有显著的抑制增殖和促进凋亡的双重调节作用。
목적：연구인21．5kDa인뇌수초감성단백（MBP）기인침묵대신경효질류U251세포증식여조망적영향。방법장21．5kDaMBP서렬특이성단발협RNA（shRNA）중조질립pGenesil‐1‐MBP‐3전염신경효질류세포주（U251）작위MBP기인침묵조，이공질립전염위음성대조조，지질체전염위지질체공전조；용실시정량PCR（RT‐PCR）화Westernblot방법검측각조21．5kDaMBPmRNA화단백적표체수평，CCK‐8법측정세포증식곡선，류식세포법분석세포조망솔。결과여음성대조조상비，MBP기인침묵조세포중21．5kDaMBP재mRNA화단백수평적표체균현저하강（P＜0．05）、세포증식활성명현강저（P＜0．05）、세포조망솔현저증고（P＜0．05）。결론인21．5kDaMBP기인침묵대신경효질류세포U251구유현저적억제증식화촉진조망적쌍중조절작용。
Objective To investigate the effects of silencing of the human cerebral 21 .5 kDa myelin basic protein (MBP) gene on the proliferation and apoptosis of the glioma U251 cells .Methods The 21 .5 kDa MBP sequence‐specific short hair‐pin RNA (shR‐NA) recombinant plasmids pGenesil‐1‐MBP‐3 were transfected into the human glioma cell line(U251) ,the cells of U251 was used as MBP silencing group ,the cells transfected with negative control plasmids used as negative control group ,and the cells transfected with liposomes used as blank control group .Real‐time PCR and Westernblot were used to detect the expression levels of the 21 .5 kDa MBP mRNA and protein in each group ,and the cell proliferation curve was measured by CCK‐8 assay ,the apoptosis rate was a‐nalysised by Flow cytometry .Results Both the mRNA and the protein expression levels of the 21 .5 kDa MBP of MBP silencing group were significantly lower than those in the control groups (P<0 .05);the cellular proliferation activity of the MBP silencing group decreased significantly (P<0 .05)while the cellular apoptotic rate increased significantly (P<0 .05) .Conclusion Silencing of the human cerebral 21 .5 kDa MBP gene may playa dual role in the inhibition of proliferation and the promotion of apoptosis of the glioma U251 cells .