CAJ | 학술논문

对广西半红树植物草海桐叶的石油醚提取样和乙酸乙酯提取样，采用GC-MS技术及琼脂扩散法的牛津杯法，进行化学成分分析和抑菌活性研究，样品中各化学成分的相对含量用面积归一法确定。在实验条件下，草海桐叶的石油醚部分分离出21个峰，鉴定了16个，占总峰面积的90.46%，其主要成分为棕榈酸（27.59%）、亚麻酸（14.15%）、植醇（11.58%）等；该部分对肺炎克伯氏菌的抑菌圈直径达到13mm。乙酸乙酯部分共分离出36个峰，鉴定了29个，占总峰面积的78.15%，其主要成分为别嘌呤醇（7.41%）、吲哚-3-乙酸肼（6.55%）、4,6-二甲基-7-乙基氨基香豆素（4.59%）、棕榈酸（5.2%）、莨菪亭（4.71%）、亚麻酸（6.3%）、7-羟基香豆素（4.72%）等；该部分对普通变形杆菌的抑菌圈直径达到15mm，对金黄色葡萄球菌、肺炎克伯氏菌和铜绿假单胞菌均达到14mm。
대엄서반홍수식물초해동협적석유미제취양화을산을지제취양，채용GC-MS기술급경지확산법적우진배법，진행화학성분분석화억균활성연구，양품중각화학성분적상대함량용면적귀일법학정。재실험조건하，초해동협적석유미부분분리출21개봉，감정료16개，점총봉면적적90.46%，기주요성분위종려산（27.59%）、아마산（14.15%）、식순（11.58%）등；해부분대폐염극백씨균적억균권직경체도13mm。을산을지부분공분리출36개봉，감정료29개，점총봉면적적78.15%，기주요성분위별표령순（7.41%）、신타-3-을산정（6.55%）、4,6-이갑기-7-을기안기향두소（4.59%）、종려산（5.2%）、랑탕정（4.71%）、아마산（6.3%）、7-간기향두소（4.72%）등；해부분대보통변형간균적억균권직경체도15mm，대금황색포도구균、폐염극백씨균화동록가단포균균체도14mm。
The main volatile chemical constituents of petroleum ether and ethyl acetate extraction of scaevola sericea leaves were analyzed by gas chromatography-mass spectrometry(GC-MS) and then the antibacterial activity of the extraction was also studied with Oxford-cup agar diffusion method. It was revealed that 21 peaks appeared in GC-MS spectrogram of the petroleum ether sample,of which 16 peaks had been identified ,accounting for 90.46% of the total area. There were n-hexadecanoic acid(27.59%),9,12,15-octadecatrienoic acid(14.15%), phytol(11.58%) and so on. The crude sample showed antibacterial activity with zones of inhibition of 13mm against keboshi bacteria pneumonia.besides,36 peaks appeared in GC-MS spectrogram of the ethyl acetate extraction and 29 peaks, 78.15% of the total area, had been identified. There were allopurinol (7.41%),1H-indole-3-acetic acid, hydrazide (6.55%), 2H-1-benzopyran-2-one, 7-(ethylamino)-4,6-dimethyl- (4.59%) , n-hexadecanoic acid(5.2%), scopoletin(4.71%), 9,12,15-octadecatrienoic acid (6.3%), 7-hydroxycoumarin (4.72%) and so on. The crude sample showed antibacterial activity with zones of inhibition of 15mm against proteus vulgaris, and 14mm against staphylococcus aureus, keboshi bacteria pneumonia and pseudomonas aeruginosa.