CAJ | 학술논문

目的：鉴定与癌源性免疫球蛋白G（IgG）全分子相互作用的蛋白质，为研究其生物功能提供重要的线索。方法分别用兔抗人IgG全分子抗体和兔IgG在宫颈癌细胞系HeLa中进行免疫共沉淀，将得到的免疫复合物经电泳分离后进行银染。经比较分析后3条差异条带切下来做质谱鉴定，质谱数据经Swiss-Prot数据库分析。最后对所得的蛋白质进行筛选和功能注释分析。结果最终得到6个可能与癌源性IgG全分子相互作用的蛋白质。结论通过免疫共沉淀结合质谱分析的方法最终得到了6个可能与癌源性IgG全分子相互作用的蛋白质，为其功能研究提供了重要线索。
목적：감정여암원성면역구단백G（IgG）전분자상호작용적단백질，위연구기생물공능제공중요적선색。방법분별용토항인IgG전분자항체화토IgG재궁경암세포계HeLa중진행면역공침정，장득도적면역복합물경전영분리후진행은염。경비교분석후3조차이조대절하래주질보감정，질보수거경Swiss-Prot수거고분석。최후대소득적단백질진행사선화공능주석분석。결과최종득도6개가능여암원성IgG전분자상호작용적단백질。결론통과면역공침정결합질보분석적방법최종득도료6개가능여암원성IgG전분자상호작용적단백질，위기공능연구제공료중요선색。
Objective To identify cancer-derived immunoglobulin G (IgG) whole molecule-interacting proteins to provide important clues for studying IgG biological functions. Methods HeLa cell lysate was immunoprecipitated with rabbit antihuman IgG whole molecule antibody and normal rabbit IgG. The immunocomplex underwent sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and was detected with silver staining. Three prominently enhanced bands were subjected to protein identification with liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the MS data were analyzed with Swiss-Prot database. Cancer-derived IgG whole molecule-interacting proteins were screened and functionally annotated. Results and Conclusion We identified 6 potential cancer-derived IgG whole molecule-interacting proteins with co-immunoprecipitation combined with LC-MS/MS, which provides valuable clues for studying the function of cancer-derived IgG.