CAJ | 학술논문

目的：探讨子痫前期患者胎盘Foxp3的表达变化和Foxp3-924（rs2232365）基因位点多态性与子痫前期的相关性。方法选取2010年10月~2011年12月在广州医医科大学附属第三医院产科住院的子痫前期患者156例，和同期住院分娩的正常妊娠孕妇252例，应用PCR-SSP技术进行Foxp3-924（rs2232365）基因位点分型。在上述对象中随机选择子痫前期患者68例，其中轻度痫前期患者33例，重度痫前期患者35例，正常晚期妊娠患者30例，采用免疫组织化学法检测蜕膜Foxp3的表达。结果Foxp3在轻度子痫前期和重度子痫前期组的表达阳性率分别为51.52%和28.57%，显著低于正常妊娠组（86.67%，P<0.05）；子痫前期的Foxp3-924G/G、G/A、A/A表型频率分别为：0.1346、0.4615、0.4038，对照组分别为0.1508、0.4087、0.4405；子痫前期组Foxp3-924 A、Foxp3-924 G基因频率分别为：0.6346和0.3654，对照组分别为：0.6448和0.3552；Foxp3-924各种基因型频率在子痫前期组与正常对照组之间差异无统计学意义（P>0.05）。结论子痫前期患者胎盘组织中的Foxp3表达量显著性低于正常孕妇，提示Foxp3表达量的水平下降使其免疫抑制功能减弱，母胎免疫耐受失衡，诱导子痫前期的发生；Foxp3-924（rs2232365）位点基因多态性对子痫前期发生无显著性作用与影响。
목적：탐토자간전기환자태반Foxp3적표체변화화Foxp3-924（rs2232365）기인위점다태성여자간전기적상관성。방법선취2010년10월~2011년12월재엄주의의과대학부속제삼의원산과주원적자간전기환자156례，화동기주원분면적정상임신잉부252례，응용PCR-SSP기술진행Foxp3-924（rs2232365）기인위점분형。재상술대상중수궤선택자간전기환자68례，기중경도간전기환자33례，중도간전기환자35례，정상만기임신환자30례，채용면역조직화학법검측세막Foxp3적표체。결과Foxp3재경도자간전기화중도자간전기조적표체양성솔분별위51.52%화28.57%，현저저우정상임신조（86.67%，P<0.05）；자간전기적Foxp3-924G/G、G/A、A/A표형빈솔분별위：0.1346、0.4615、0.4038，대조조분별위0.1508、0.4087、0.4405；자간전기조Foxp3-924 A、Foxp3-924 G기인빈솔분별위：0.6346화0.3654，대조조분별위：0.6448화0.3552；Foxp3-924각충기인형빈솔재자간전기조여정상대조조지간차이무통계학의의（P>0.05）。결론자간전기환자태반조직중적Foxp3표체량현저성저우정상잉부，제시Foxp3표체량적수평하강사기면역억제공능감약，모태면역내수실형，유도자간전기적발생；Foxp3-924（rs2232365）위점기인다태성대자간전기발생무현저성작용여영향。
Objective To detect changes of Foxp3 expression in the decidua in patients with preeclampsia and investigate the correlation of Foxp3-924 (rs2232365) polymorphisms with preeclampsia. Methods From October 2011 to December 2012, 252 normal pregnant women and 156 preeclampsia patients of Han nationality from the same geographic region were tested for Foxp3-924 genotypes by polymerase chain reaction with sequence-specific primer (PCR-SSP). Sixty-eight of the patients with preeclampsia (33 with mild and 35 with severe preeclampsia) and 30 of the normal pregnant women were also examined for Foxp3 expression in the decidua using immunohistochemical method. Results Foxp3 positive expression rates in the decidua was 51.52%in mild preeclampsia and 28.57%in severe preeclampsia cases, significantly lower than that in the control group (86.67%, P<0.05). In preeclampsia patients, the frequencies of Foxp3-924G/G, G/A, and A/A genotypes were 0.1346, 0.4615 and 0.4038, respectively, and the frequencies of Foxp3-924A and Foxp3-924 G were 0.6346 and 0.3654, respectively. The genotype frequencies of Foxp3-924G/G, G/A and A/A in the control group were 0.1508, 0.4087 and 0.4405, respectively, and the frequencies of Foxp3-924 A and Foxp3-924 G were 0.6448 and 0.3552, respectively. No significant differences were found in the gene frequencies of Foxp3-924G/A between preeclampsia patients and the control group (P>0.05). Conclusion The expression level of Foxp3 in the placental tissue of preeclampsia patients is significantly lower than that in normal pregnant women, suggesting that lowered Foxp3 expression decreases the immunosuppressive function and causes imbalance of immune tolerance between maternal-fetal to induce preeclampsia. Foxp3-924 polymorphisms is not significantly correlated with the occurrence of preeclampsia.