CAJ | 학술논문

目的：研究BAMBI基因在小鼠不同脂肪组织发育过程中的表达规律。方法利用RT-qPCR及Western blot技术检测BAMBI基因在小鼠不同类型脂肪组织、不同发育阶段（胚胎第18天、出生第0天、第21天、第8周和第6个月）及原代前体脂肪细胞分化过程中（分化第0、3、7、11天）的差异表达情况。结果随着脂肪组织的发育，BAMBI mRNA及蛋白的表达量逐渐降低；在发育的同一时间段，BAMBI mRNA在内脏白色脂肪组织较皮下白色脂肪组织表达量高，在棕色脂肪组织中表达量最低；在小鼠前体脂肪细胞诱导分化过程中，BAMBI mRNA表达量呈时间依赖性降低。结论提示BAMBI的差异表达规律与脂肪组织类型、发育阶段以及脂肪细胞分化程度密切相关，其在脂肪组织发育过程中起着重要的作用。
목적：연구BAMBI기인재소서불동지방조직발육과정중적표체규률。방법이용RT-qPCR급Western blot기술검측BAMBI기인재소서불동류형지방조직、불동발육계단（배태제18천、출생제0천、제21천、제8주화제6개월）급원대전체지방세포분화과정중（분화제0、3、7、11천）적차이표체정황。결과수착지방조직적발육，BAMBI mRNA급단백적표체량축점강저；재발육적동일시간단，BAMBI mRNA재내장백색지방조직교피하백색지방조직표체량고，재종색지방조직중표체량최저；재소서전체지방세포유도분화과정중，BAMBI mRNA표체량정시간의뢰성강저。결론제시BAMBI적차이표체규률여지방조직류형、발육계단이급지방세포분화정도밀절상관，기재지방조직발육과정중기착중요적작용。
Objective To investigate the expression profiles of bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) during the development of mouse adipose tissue. Methods The total RNA was extracted for real-time PCR for amplification of BAMBI mRNA from the suprascapular brown adipose tissue (BAT) and subcutaneous (inguinal) and visceral (gonadal) white adipose tissue (sWAT and vWAT, respectively) of mice at various embryonic and postnatal stages, as well as from isolated primary preadipocytes during differentiation. Results In BAT, BAMBI mRNA levels exhibited a transient increase, peaking at day 0 (D0) and declined thereafter. sWAT and vWAT could be isolated from mice from postnatal D21 onwards, in which BAMBI mRNA levels were the highest and decreased at 8 weeks and 6 months. BAMBI mRNA levels were also significantly reduced in primary preadipocytes isolated from vWAT after induced differentiation. BAMBI mRNA expression level was higher in vWAT than in sWAT and BAT at the same developmental stages. Conclusion BAMBI is differentially expressed in different adipose tissues and developmental stages, which supports the hypothesis that BAMBI plays a pivotal role in the development of adipose tissues.