西部中医药
西部中醫藥
서부중의약
GANSU JOURNAL OF TRADITIONAL CHINESE MEDICINE
2015年
1期
23-25
,共3页
强力感冒片%绿原酸%高效液相色谱法%指纹图谱
彊力感冒片%綠原痠%高效液相色譜法%指紋圖譜
강력감모편%록원산%고효액상색보법%지문도보
QiangLiGanMaotablets%Chlorogenic acid%HPLC%fingerprint
目的：研究强力感冒片的HLPC指纹图谱。方法：采用HLPC方法测定，色谱柱：KromasilODS-2 C18(250 mm×4.6 mm,5μm)；检测波长：327 nm；流速：1.0 mL/min；柱温：25℃；乙腈-0.4%的磷酸水溶液(12：88)为流动相。结果：确定了7个共有峰，5批样品每批处理两份，10份样品指纹图谱的相似度均在0.9以上，绿原酸的线性范围为0.03～0.33μg/mL,加样回收率为101.27%。结论：所建立的HLPC简便可靠，可用于强力感冒片的质量控制。
目的：研究彊力感冒片的HLPC指紋圖譜。方法：採用HLPC方法測定，色譜柱：KromasilODS-2 C18(250 mm×4.6 mm,5μm)；檢測波長：327 nm；流速：1.0 mL/min；柱溫：25℃；乙腈-0.4%的燐痠水溶液(12：88)為流動相。結果：確定瞭7箇共有峰，5批樣品每批處理兩份，10份樣品指紋圖譜的相似度均在0.9以上，綠原痠的線性範圍為0.03～0.33μg/mL,加樣迴收率為101.27%。結論：所建立的HLPC簡便可靠，可用于彊力感冒片的質量控製。
목적：연구강력감모편적HLPC지문도보。방법：채용HLPC방법측정，색보주：KromasilODS-2 C18(250 mm×4.6 mm,5μm)；검측파장：327 nm；류속：1.0 mL/min；주온：25℃；을정-0.4%적린산수용액(12：88)위류동상。결과：학정료7개공유봉，5비양품매비처리량빈，10빈양품지문도보적상사도균재0.9이상，록원산적선성범위위0.03～0.33μg/mL,가양회수솔위101.27%。결론：소건립적HLPC간편가고，가용우강력감모편적질량공제。
Objective: To investigate the fingerprints ofQiangLiGanMaotablets by HPLC. Methods: The HPLC method was adopted to detect with the conditions of chromatographic column being KromasilODS-2 C18 (250 mm×4.6 mm, 5μm), detection wavelength 327 nm, flow speed 1.0 mL/min, column temperature 25℃as well as mobile phase being CH3CN - 0.4% H3PO4(12/88). Results: Seven common peaks consisted in the fingerprints of five batches of samples which were divided into two groups. Finally, the similarity of ten fingerprints was over 0.9, the linear range of chlorogenic acid was from 0.03 ug to 0.33μg/mL and the recovery of chlorogenic acid was 101.27%. Conclusion: The HPLC method applied is simple, convenient and reliable and can be used to control the quality of QiangLi GanMao tablets.
