CAJ | 학술논문

目的：观察“从肠论治”对哮喘小鼠支气管肺泡灌洗液中嗜酸性粒细胞、中性粒细胞及血清IgE含量的影响，为探讨“肺与大肠相表里”理论提供实验依据。方法：采用卵清蛋白（ OVA）致敏激发方法制备过敏性哮喘小鼠模型，芒硝灌胃刺激，进行小鼠气道反应性试验，肺组织形态学观察，肺支气管肺泡灌洗液细胞分类计数、血清IgE含量检测。结果：与正常组相比，OVA组小鼠气道阻力增加，肺组织出现炎性病理改变，肺泡灌洗液中嗜酸性粒细胞、淋巴细胞总数明显增加，血清IgE含量均明显升高；经芒硝从肠干预后，芒硝组小鼠气道阻力明显降低，肺组织形态结构有一定程度的改善，肺泡灌洗液中嗜酸性粒细胞、淋巴细胞总数明显减少，血清IgE含量显著降低。结论：芒硝刺激大肠，有助于改善气道通气功能，抑制哮喘小鼠气道炎症，改善气道免疫微环境。
목적：관찰“종장론치”대효천소서지기관폐포관세액중기산성립세포、중성립세포급혈청IgE함량적영향，위탐토“폐여대장상표리”이론제공실험의거。방법：채용란청단백（ OVA）치민격발방법제비과민성효천소서모형，망초관위자격，진행소서기도반응성시험，폐조직형태학관찰，폐지기관폐포관세액세포분류계수、혈청IgE함량검측。결과：여정상조상비，OVA조소서기도조력증가，폐조직출현염성병리개변，폐포관세액중기산성립세포、림파세포총수명현증가，혈청IgE함량균명현승고；경망초종장간예후，망초조소서기도조력명현강저，폐조직형태결구유일정정도적개선，폐포관세액중기산성립세포、림파세포총수명현감소，혈청IgE함량현저강저。결론：망초자격대장，유조우개선기도통기공능，억제효천소서기도염증，개선기도면역미배경。
Objective:To explore the biological foundation for the TCM theory of the interior-exterior relationship between the lung and the large intestine, we conducted an experiment to evaluate the content of eosinophils and neutrophils in bronchoalveolar lavage fluid ( BALF) and IgE in blood serum after the asthma modeled mice were intervened with mirabilite at large intestines.Methods:Asthma model mice were established by OVA sensitization.Airway responsiveness, histopathology change, classification and counting of the cells of BALF and content of blood serum IgE were observed after large intestine was stimulated by mirabilite.Re-sults:Compared with the normal rats, the asthma model rats’airway resistance increased obviously, the number of eosinophils and neutrophils in BALF and IgE in blood serum significantly increased.After intervened with mirabilite, airway resistance decreased obviously, eosinophils and neutrophils in BALF and IgE in blood serum significantly lowered.Conclusion: Stimulating the large intestine by mirabilite showed beneficial effects in alleviating airway responsiveness and airway inflammation, and improving airway immune microenvironment in asthma mice model.