世界中医药
世界中醫藥
세계중의약
WORLD CHINESE MEDICINE
2015年
1期
83-85
,共3页
半枝莲提取物%黄曲霉素B1%肝癌%Notch1
半枝蓮提取物%黃麯黴素B1%肝癌%Notch1
반지련제취물%황곡매소B1%간암%Notch1
Banzhilian%Aflatoxin B1%Hepatic carcinoma%Notch1
目的：探讨半枝莲提取物（ ESB）对黄曲霉素B1所诱发的肝癌大鼠Notch1的影响。方法：选取60只Wistar大鼠，随机分为空白组、模型组、半枝莲提取物低、高剂量组，每组15只。建立用黄曲霉素B1所诱发的肝癌大鼠的动物模型。其中空白组与模型组给予蒸馏水灌胃，半枝莲提取物低、高剂量组给予不同浓度的ESB灌胃治疗。灌胃1次／d，连续治疗1个月。治疗结束后将所有大鼠处死，观察各组大鼠的体质量、肝表面的结节数，并取肝组织采用免疫组化法检测肝组织中Notch1的表达情况。结果：与空白组比较，模型组和ESB低、高剂量组大鼠的体质量明显降低（ P＜0．05），肝表面的结节数明显增多（P＜0．05），Notch1阳性细胞数明显升高（P＜0．05）；与模型组比较，ESB低、高剂量组大鼠的体重升高（P＜0．05），肝表面的结节数及Notch1阳性细胞数明显降低（P＜0．05）。结论：低、高剂量的半枝莲提取物能够有效的抑制肝癌的恶化，其机制可能与下调肝组织中Notch1有关。
目的：探討半枝蓮提取物（ ESB）對黃麯黴素B1所誘髮的肝癌大鼠Notch1的影響。方法：選取60隻Wistar大鼠，隨機分為空白組、模型組、半枝蓮提取物低、高劑量組，每組15隻。建立用黃麯黴素B1所誘髮的肝癌大鼠的動物模型。其中空白組與模型組給予蒸餾水灌胃，半枝蓮提取物低、高劑量組給予不同濃度的ESB灌胃治療。灌胃1次／d，連續治療1箇月。治療結束後將所有大鼠處死，觀察各組大鼠的體質量、肝錶麵的結節數，併取肝組織採用免疫組化法檢測肝組織中Notch1的錶達情況。結果：與空白組比較，模型組和ESB低、高劑量組大鼠的體質量明顯降低（ P＜0．05），肝錶麵的結節數明顯增多（P＜0．05），Notch1暘性細胞數明顯升高（P＜0．05）；與模型組比較，ESB低、高劑量組大鼠的體重升高（P＜0．05），肝錶麵的結節數及Notch1暘性細胞數明顯降低（P＜0．05）。結論：低、高劑量的半枝蓮提取物能夠有效的抑製肝癌的噁化，其機製可能與下調肝組織中Notch1有關。
목적：탐토반지련제취물（ ESB）대황곡매소B1소유발적간암대서Notch1적영향。방법：선취60지Wistar대서，수궤분위공백조、모형조、반지련제취물저、고제량조，매조15지。건립용황곡매소B1소유발적간암대서적동물모형。기중공백조여모형조급여증류수관위，반지련제취물저、고제량조급여불동농도적ESB관위치료。관위1차／d，련속치료1개월。치료결속후장소유대서처사，관찰각조대서적체질량、간표면적결절수，병취간조직채용면역조화법검측간조직중Notch1적표체정황。결과：여공백조비교，모형조화ESB저、고제량조대서적체질량명현강저（ P＜0．05），간표면적결절수명현증다（P＜0．05），Notch1양성세포수명현승고（P＜0．05）；여모형조비교，ESB저、고제량조대서적체중승고（P＜0．05），간표면적결절수급Notch1양성세포수명현강저（P＜0．05）。결론：저、고제량적반지련제취물능구유효적억제간암적악화，기궤제가능여하조간조직중Notch1유관。
Objective:To investigate the Banzhilian extract’s effects on Notch1 of rats with aflatoxin B1-induced hepatic carcino-ma.Methods:Sixty Wistar rats were randomly divided into 4 groups:normal control group,hepatocarcinoma model group,extract’ s high dose group and low dose group.Hepatic carcinoma model were induced by aflatoxin B1.The control group and the model group were given distilled water lavagely, the extract ’s groups were given different concentrations of extracts lavagely, qd.for a month.All rats were killed in the 4th week,measure weight,counted number of nodules on the surface of liver.Immunohistochemis-try were used to detect the expression of Notch1.Results:Compared with the control group,the rats’weight in other groups dropped significantly ( P<0.05) ,while the nodule number and Notch1 levels increased significantly ( P<0.05);Compared with the mod-el group,the rats’weight in the extract’s of high dose group and low dose group were increased ( P<0.05) ,while the nodule num-ber and Notch1 levels were reduced considerably(P<0.05).Conclusion:Banzhilian extract could inhibit aggravation of liver car-cinoma,which is probably related to the down-regulating of liver Notch1.
