CAJ | 학술논문

在干旱和水杨酸（salicylic acid， SA）胁迫下，检测欧李叶片中交替氧化酶（alternative oxidase， AOX）及解偶联蛋白（ uncoupling protein ， UCP）基因家族的表达情况，以期为两基因家族在胁迫下的作用提供基础研究的依据。通过使用特异性引物和SYBR GreenⅠ，结合染料实时荧光定量 RT－PCR 技术，以管家基因actin基因为参照基因，采用相对定量的方法，对欧李叶片AOX家族及UCP家族进行差异表达分析。研究结果表明，干旱胁迫时， AOX4、 UCP3、 UCP4和UCP5在8天时表达量最大，之后有所下降； AOX1b、 UCP1和UCP2表达量不断升高直到12天达到峰值，而AOX1a和AOX2的变化并不明显。 SA处理3天时，仅UCP2的表达量有所上升，其他AOX家族和UCP家族成员的表达量都不同程度下降。
재간한화수양산（salicylic acid， SA）협박하，검측구리협편중교체양화매（alternative oxidase， AOX）급해우련단백（ uncoupling protein ， UCP）기인가족적표체정황，이기위량기인가족재협박하적작용제공기출연구적의거。통과사용특이성인물화SYBR GreenⅠ，결합염료실시형광정량 RT－PCR 기술，이관가기인actin기인위삼조기인，채용상대정량적방법，대구리협편AOX가족급UCP가족진행차이표체분석。연구결과표명，간한협박시， AOX4、 UCP3、 UCP4화UCP5재8천시표체량최대，지후유소하강； AOX1b、 UCP1화UCP2표체량불단승고직도12천체도봉치，이AOX1a화AOX2적변화병불명현。 SA처리3천시，부UCP2적표체량유소상승，기타AOX가족화UCP가족성원적표체량도불동정도하강。
In order to provide fundamental knowlege of genes from alternative oxidase ( AOX ) and uncoupling protein( UCP) families, the expression levels of them were investigated in Prunus humilis leaves under water stress(WS)and salicylic acid(SA)treatments in the present study.Specific primers and real-time fluorescence quantitative RT -PCR were designed and carried out .The actin gene , a housekeeping gene was used as reference gene .Then, we assayed the changes of AOX and UCP families in the leaves of Prunus humilis using the method of relative quantification PCR . As a result , the expression level of AOX 4 , UCP3 , UCP4 and UCP5 exhibited a maximum value on the day 8 under WS , thereafter, it declined gradualy.However, the expression level of AOX1b, UCP1 and UCP2 increased over time, and reached the peak on the day 12.No changes of AOX1a and AOX2 were observed compared with controls . On the day 3 of SA treatment , the expression levels of all members of both families were declined except UCP2.