CAJ | 학술논문

目的：通过实时荧光定量PCR（RT-PCR）法和 ELISA法检测患者体液中 HCMV-DNA及 HCMV-IgM，比较这两种方法检测的阳性率和推测其诊断人巨细胞病毒感染的价值。方法441名患者的标本用 RT-PCR法检测HCMV-DNA，用 ELISA检测 HCMV-IgM，计算两种方法的阳性率。结果两种方法单独检测的阳性率分别为26.08％和17．46％，两者比较，差异有统计学意义（χ2＝26．74，P＜0．005），RT-PCR法的阳性率高于ELISA法。两种方法联合检测的阳性率为27．89％（123／441），高于两方法单独检测阳性率。结论用 RT-PCR法检测尿液或血液标本 HCMV-DNA与 ELISA法检测血清 HCMV-IgM联合应用提高了患者 HCMV感染检测的阳性率，减少了漏诊率。
목적：통과실시형광정량PCR（RT-PCR）법화 ELISA법검측환자체액중 HCMV-DNA급 HCMV-IgM，비교저량충방법검측적양성솔화추측기진단인거세포병독감염적개치。방법441명환자적표본용 RT-PCR법검측HCMV-DNA，용 ELISA검측 HCMV-IgM，계산량충방법적양성솔。결과량충방법단독검측적양성솔분별위26.08％화17．46％，량자비교，차이유통계학의의（χ2＝26．74，P＜0．005），RT-PCR법적양성솔고우ELISA법。량충방법연합검측적양성솔위27．89％（123／441），고우량방법단독검측양성솔。결론용 RT-PCR법검측뇨액혹혈액표본 HCMV-DNA여 ELISA법검측혈청 HCMV-IgM연합응용제고료환자 HCMV감염검측적양성솔，감소료루진솔。
Objective To detect HCMV viral load in body fluids and HCMV-IgM in the serum and to study the clinical value of the combined detection for the patients with human cytomegalovirus infection.Methods In 441 pa-tients,HCMV-IgM in the serum was detected by ELISA and HCMV-DNA viral load in body fluids was detected by RT-PCR,compare with these two kinds of methods to detect the positive rate and the diagnostic value.Results The positive rate of two methods were 26.08% and 17.46% respectively,the significance and the difference between two methods was obviously(χ2=26.74,P<0.005).The positive rate of combined detection by two methods was 27.89%(123/441),which was higher than that of the single.Conclusion The combined application of HCMV-IgM in the ser-um using ELISA and the copies of HCMV-DNA in urine using RT-PCR would raise the positive rate and reduce the missed diagnosis of detection for the patients with the human cytomegalovirus infection.