中国实验诊断学
中國實驗診斷學
중국실험진단학
CHINESE JOURNAL OF LABORATORY DIAGNOSIS
2015年
1期
50-53
,共4页
孙牧男%刘磊%马寅芙%许淑芬%方艳秋%谭岩
孫牧男%劉磊%馬寅芙%許淑芬%方豔鞦%譚巖
손목남%류뢰%마인부%허숙분%방염추%담암
肺癌%CXCL12%CXCR4
肺癌%CXCL12%CXCR4
폐암%CXCL12%CXCR4
small cell lung cancer%CXCL1 2%CXCR4
目的：研究小细胞肺癌（small cell lung cancer，SCLC）患者外周血中 CXCL12及其受体 CXCR4的表达情况，探讨CXCL12／CXCR4与SCLC发生和发展之间的关系。方法采用ELISA、real-time PCR和Westernblot方法，检测CXCL12与CXCR4在30例局限期（Limited stage disease，LD）SCLC患者、32例扩散期（Expensive stage disease， ED）SCLC患者和10例良性肺部肿瘤患者（对照组，Control）中的转录水平和表达水平，分析 CXCL12、CXCR4与SCLC患者临床病理等指标之间的关系。结果 CXCL12、CXCR4在 SCLC患者组织中均高表达，其中扩散期患者CXCL12、CXCR4表达平略高于局限期患者组。扩散期患者外周血中 CXCL12表达高于其在局限期患者中的水平，二者均高于对照组。血清中CXCL12水平与患者的性别和年龄无关（P＞0．05），而与肿瘤的大小及淋巴结转移明显相关（P＜0．01）。结论 CXCL12／CXCR4可能直接或间接参与对 SCLC发生和发展的调控。
目的：研究小細胞肺癌（small cell lung cancer，SCLC）患者外週血中 CXCL12及其受體 CXCR4的錶達情況，探討CXCL12／CXCR4與SCLC髮生和髮展之間的關繫。方法採用ELISA、real-time PCR和Westernblot方法，檢測CXCL12與CXCR4在30例跼限期（Limited stage disease，LD）SCLC患者、32例擴散期（Expensive stage disease， ED）SCLC患者和10例良性肺部腫瘤患者（對照組，Control）中的轉錄水平和錶達水平，分析 CXCL12、CXCR4與SCLC患者臨床病理等指標之間的關繫。結果 CXCL12、CXCR4在 SCLC患者組織中均高錶達，其中擴散期患者CXCL12、CXCR4錶達平略高于跼限期患者組。擴散期患者外週血中 CXCL12錶達高于其在跼限期患者中的水平，二者均高于對照組。血清中CXCL12水平與患者的性彆和年齡無關（P＞0．05），而與腫瘤的大小及淋巴結轉移明顯相關（P＜0．01）。結論 CXCL12／CXCR4可能直接或間接參與對 SCLC髮生和髮展的調控。
목적：연구소세포폐암（small cell lung cancer，SCLC）환자외주혈중 CXCL12급기수체 CXCR4적표체정황，탐토CXCL12／CXCR4여SCLC발생화발전지간적관계。방법채용ELISA、real-time PCR화Westernblot방법，검측CXCL12여CXCR4재30례국한기（Limited stage disease，LD）SCLC환자、32례확산기（Expensive stage disease， ED）SCLC환자화10례량성폐부종류환자（대조조，Control）중적전록수평화표체수평，분석 CXCL12、CXCR4여SCLC환자림상병리등지표지간적관계。결과 CXCL12、CXCR4재 SCLC환자조직중균고표체，기중확산기환자CXCL12、CXCR4표체평략고우국한기환자조。확산기환자외주혈중 CXCL12표체고우기재국한기환자중적수평，이자균고우대조조。혈청중CXCL12수평여환자적성별화년령무관（P＞0．05），이여종류적대소급림파결전이명현상관（P＜0．01）。결론 CXCL12／CXCR4가능직접혹간접삼여대 SCLC발생화발전적조공。
Objective To study the expression levels of CXCL12 and CXCR4 in patients with small cell lung cancer (SCLC),and to investigate the correlation between CXCL1 2/CXCR4 and the development of human SCLC.Methods The serum concentration of CXCL1 2 was detected by ELISA,and the expression of CXCL1 2/CXCR4 in tissues were detected by real-time PCR and Westernblot respectively in 30 SCLC patients in limited stage,32 SCLC patients in ex-tensive stage and 10 benign lung cancers (control).Then we analyzed the correlation between CXCL12/CXCR4 and clinical pathological characteristics of SCLC.Results The concentration of serum CXCL1 2 was higher in patients with limited stage than that in patients with extensive stage,both of them were showed a higher level than that in HC.The expressions of CXCL12/CXCR4 were also higher in SCLC patients than that in HC as well.The serum level of CXCL12 was associated with tumor size and lymphatic metastasis (P<0.01)and had no relations with sex or age of patients (P>0.05).Conclusion The expression of CXCL12/CXCR4 may participate in regulating the development of SCLC.
