CAJ | 학술논문

目的：探讨实时荧光定量聚合酶链反应技术（RT-PCR）对结核性脑膜炎的诊断价值。方法分别采用涂片法、RT-PCR法对结核性脑膜炎、疑似结核性脑膜炎以及非结核性脑膜炎患者的脑脊液标本进行检测，并对检测结果进行比较分析。结果涂片法共检出10例阳性，其中结核性脑膜炎7例（70．00％），疑似结核性脑膜炎3例（30.00％）；RT-PCR法共检出46例阳性，其中结核性脑膜炎31例（67．39％），疑似结核性脑膜炎15例（32．61％）。对结核性脑膜炎患者检测，涂片法阳性率（12．28％）低于 RT-PCR 法阳性率（54．39％），差异有统计学意义（P＜0.05）；对疑似结核性脑膜炎患者检测，涂片法阳性率（2．91％）低于 RT-PCR法阳性率（14．56％），差异有统计学意义（P＜0．05）；脑脊液涂片法检测结核性脑膜炎的灵敏度为12．28％，漏诊率为87．72％，RT-PCR法检测的灵敏度为54．39％，漏诊率为45．61％，差异有统计学意义（P＜0．05）。结论 RT-PCR法诊断结核性脑膜炎具有灵敏度好，准确度高等特点，临床有重要的指导意义。
목적：탐토실시형광정량취합매련반응기술（RT-PCR）대결핵성뇌막염적진단개치。방법분별채용도편법、RT-PCR법대결핵성뇌막염、의사결핵성뇌막염이급비결핵성뇌막염환자적뇌척액표본진행검측，병대검측결과진행비교분석。결과도편법공검출10례양성，기중결핵성뇌막염7례（70．00％），의사결핵성뇌막염3례（30.00％）；RT-PCR법공검출46례양성，기중결핵성뇌막염31례（67．39％），의사결핵성뇌막염15례（32．61％）。대결핵성뇌막염환자검측，도편법양성솔（12．28％）저우 RT-PCR 법양성솔（54．39％），차이유통계학의의（P＜0.05）；대의사결핵성뇌막염환자검측，도편법양성솔（2．91％）저우 RT-PCR법양성솔（14．56％），차이유통계학의의（P＜0．05）；뇌척액도편법검측결핵성뇌막염적령민도위12．28％，루진솔위87．72％，RT-PCR법검측적령민도위54．39％，루진솔위45．61％，차이유통계학의의（P＜0．05）。결론 RT-PCR법진단결핵성뇌막염구유령민도호，준학도고등특점，림상유중요적지도의의。
Objective To investigate the diagnosis value of real-time fluorescent quantitative polymerase chain reac-tion(TR-PCR)in tuberculous meningitis.Methods The cerebrospinal fluid specimens of patients with tuberculous meningitis,suspected tuberculous meningitis and non-tuberculous meningitis were detected by using smear method and RT-PCR respectively,and the testing?results were compared and analyzed.Results There were 10 cases of positive by smear method,including 7 cases of tuberculous meningitis(70.00%)and 3 cases of suspected tuberculous meningitis (30.00%);while 46 cases of positive by TR-PCR method,including 31 cases of tuberculous meningitis(67.39%)and 15 cases of suspected tuberculous meningitis(32.61%).In detection of patients with tuberculous meningitis,the posi-tive rate by smear method was 12.28%,which was lower than 54.39% by RT-PCR method,the difference was statisti-cally significance(P<0.05).In detection of patients with suspected tuberculous meningitis,the positive rate in smear method was 2.91%,which was lower than 14.56% in RT-PCR method,the difference was statistically significance(P<0.05).The sensitivity of smear method in detecting tuberculous meningitis was 12.28%,and the rate of missed diagno-sis was 87.72%,while the RT-PCR was 54.39% and 45.61% respectively,the sensitivity of TR-PCR was higher than that of smear method,the difference was statistically significance(P<0.05).Conclusion RT-PCR in diagnosis of tuberculous menin-gitis has a characteristic of good sensitivity,high accuracy,and it has an important clinical significance.