中国实验诊断学
中國實驗診斷學
중국실험진단학
CHINESE JOURNAL OF LABORATORY DIAGNOSIS
2015年
1期
14-16
,共3页
苏维奇%朱元祺%李莉%毕春霞%闫志勇%邓乃梅
囌維奇%硃元祺%李莉%畢春霞%閆誌勇%鄧迺梅
소유기%주원기%리리%필춘하%염지용%산내매
粘质沙雷菌%耐药性%整合子
粘質沙雷菌%耐藥性%整閤子
점질사뢰균%내약성%정합자
Serratia marcescens%Drug resistance%Integron
目的:了解本地区粘质沙雷菌临床分离株Ⅰ、Ⅱ、Ⅲ类整合子的携带情况,探讨整合子与其耐药表型的相关性。方法收集临床分离的经 VITEK-2 Compact 细菌分析系统鉴定的粘质沙雷菌287株,以煮沸法制备细菌DNA作为PCR扩增模板,采用聚合酶链反应(PCR)检测菌株的第Ⅰ、Ⅱ、Ⅲ类整合子,分析比较整合子阳性菌株与阴性菌株的药敏试验结果差异。结果在287株粘质沙雷菌中,有262株细菌检出Ⅰ类整合子,检出率为91.3%,3株细菌检出Ⅲ类整合子,未检出Ⅱ类整合子。药敏结果显示,第Ⅰ类整合子阳性菌株对环丙沙星和头孢噻肟的耐药率高于Ⅰ类整合子阴性菌株(P均<0.05),对其他抗菌药物的耐药性,Ⅰ类整合子阳性菌株和阴性菌株间差异无统计学意义(P均>0.05)。结论Ⅰ类整合子在粘质沙雷菌中普遍存在,Ⅰ类整合子与粘质沙雷菌的多重耐药无明显的相关性。
目的:瞭解本地區粘質沙雷菌臨床分離株Ⅰ、Ⅱ、Ⅲ類整閤子的攜帶情況,探討整閤子與其耐藥錶型的相關性。方法收集臨床分離的經 VITEK-2 Compact 細菌分析繫統鑒定的粘質沙雷菌287株,以煮沸法製備細菌DNA作為PCR擴增模闆,採用聚閤酶鏈反應(PCR)檢測菌株的第Ⅰ、Ⅱ、Ⅲ類整閤子,分析比較整閤子暘性菌株與陰性菌株的藥敏試驗結果差異。結果在287株粘質沙雷菌中,有262株細菌檢齣Ⅰ類整閤子,檢齣率為91.3%,3株細菌檢齣Ⅲ類整閤子,未檢齣Ⅱ類整閤子。藥敏結果顯示,第Ⅰ類整閤子暘性菌株對環丙沙星和頭孢噻肟的耐藥率高于Ⅰ類整閤子陰性菌株(P均<0.05),對其他抗菌藥物的耐藥性,Ⅰ類整閤子暘性菌株和陰性菌株間差異無統計學意義(P均>0.05)。結論Ⅰ類整閤子在粘質沙雷菌中普遍存在,Ⅰ類整閤子與粘質沙雷菌的多重耐藥無明顯的相關性。
목적:료해본지구점질사뢰균림상분리주Ⅰ、Ⅱ、Ⅲ류정합자적휴대정황,탐토정합자여기내약표형적상관성。방법수집림상분리적경 VITEK-2 Compact 세균분석계통감정적점질사뢰균287주,이자비법제비세균DNA작위PCR확증모판,채용취합매련반응(PCR)검측균주적제Ⅰ、Ⅱ、Ⅲ류정합자,분석비교정합자양성균주여음성균주적약민시험결과차이。결과재287주점질사뢰균중,유262주세균검출Ⅰ류정합자,검출솔위91.3%,3주세균검출Ⅲ류정합자,미검출Ⅱ류정합자。약민결과현시,제Ⅰ류정합자양성균주대배병사성화두포새우적내약솔고우Ⅰ류정합자음성균주(P균<0.05),대기타항균약물적내약성,Ⅰ류정합자양성균주화음성균주간차이무통계학의의(P균>0.05)。결론Ⅰ류정합자재점질사뢰균중보편존재,Ⅰ류정합자여점질사뢰균적다중내약무명현적상관성。
Objective Understanding of the carrying situation in the region of Serratia marcescens isolates Ⅰ,Ⅱ,Ⅲintegron,To explore the relationship between integrons and drug resistance phenotype.Methods Clinical isolates col-lected by VITEK-2 Compact analysis system for identification of 287 strains of Serratia marcescens.Prepared to boil PCR amplification of bacterial DNA as a template,using the polymerase chain reaction (PCR)testing of classⅠ,Ⅱ,Ⅲintegron.Analysis and comparison the differences of integron positive strains and negative strains in drug sensitive test. Results In 287 strains of Serratia marcescens,262 strains of bacteria detected integron,the detection rate was 91.3%, 3 strains of bacteria detected class Ⅲ integron,II integron were not detected.The drug sensitivity results showed,the classⅠ integron positive strains resistant to ciprofloxacin and cefotaxime were higher than that of Ⅰintegron negative strains (P<0.05).Resistance to other antimicrobial drugs,among Ⅰ integron positive and negative strains were no significant differences (P>0.05).Conclusion Ⅰ integron prevalent in Serratia marcescens,class Ⅰ integron and mul-tidrug-resistant Serratia marcescens is no significant correlation.