浙江中西医结合杂志
浙江中西醫結閤雜誌
절강중서의결합잡지
ZHEJIANG JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2015年
1期
17-19,108
,共4页
大鼠%糖尿病肾病%足细胞%绞股蓝总皂苷%nephrin%VEGF
大鼠%糖尿病腎病%足細胞%絞股藍總皂苷%nephrin%VEGF
대서%당뇨병신병%족세포%교고람총조감%nephrin%VEGF
rats%diabetic nephropathy%podocyte%gypenoside%nephrin%VEGF
目的：观察绞股蓝总皂苷对糖尿病肾病（DN）大鼠nephrin、VEGF表达的影响，探讨其降低尿蛋白、保护肾脏的作用机制。方法采用单侧肾切除加链脲佐菌素注射法改良复制大鼠DN模型，实验分为正常组、模型组、绞股蓝总皂苷高、中、低剂量组和缬沙坦组，每组8只。药物干预4周后测定24h尿蛋白、内生肌酐清除率、足细胞相关分子nephrin及足细胞分泌的VEGF蛋白在肾脏组织的表达。结果绞股蓝总皂苷高剂量组24h尿蛋白明显低于模型组[（77.04±16.63）mg比（110.56±46.88）mg，P<0.05]，内生肌酐清除率高于模型组[（1.47±0.24）mL·min-1比（1.25±0.23）mL· min-1，P<0.05]。高剂量组nephrin蛋白表达水平较模型组明显上调（0.4250±0.02比0.3388±0.02，P<0.01），VEGF表达明显抑制（0.2588±0.03比0.3521±0.01，P<0.01），且效果类似缬沙坦组。结论绞股蓝总皂苷能降低DN大鼠尿蛋白，改善肾功能和足细胞相关蛋白的异常表达。
目的：觀察絞股藍總皂苷對糖尿病腎病（DN）大鼠nephrin、VEGF錶達的影響，探討其降低尿蛋白、保護腎髒的作用機製。方法採用單側腎切除加鏈脲佐菌素註射法改良複製大鼠DN模型，實驗分為正常組、模型組、絞股藍總皂苷高、中、低劑量組和纈沙坦組，每組8隻。藥物榦預4週後測定24h尿蛋白、內生肌酐清除率、足細胞相關分子nephrin及足細胞分泌的VEGF蛋白在腎髒組織的錶達。結果絞股藍總皂苷高劑量組24h尿蛋白明顯低于模型組[（77.04±16.63）mg比（110.56±46.88）mg，P<0.05]，內生肌酐清除率高于模型組[（1.47±0.24）mL·min-1比（1.25±0.23）mL· min-1，P<0.05]。高劑量組nephrin蛋白錶達水平較模型組明顯上調（0.4250±0.02比0.3388±0.02，P<0.01），VEGF錶達明顯抑製（0.2588±0.03比0.3521±0.01，P<0.01），且效果類似纈沙坦組。結論絞股藍總皂苷能降低DN大鼠尿蛋白，改善腎功能和足細胞相關蛋白的異常錶達。
목적：관찰교고람총조감대당뇨병신병（DN）대서nephrin、VEGF표체적영향，탐토기강저뇨단백、보호신장적작용궤제。방법채용단측신절제가련뇨좌균소주사법개량복제대서DN모형，실험분위정상조、모형조、교고람총조감고、중、저제량조화힐사탄조，매조8지。약물간예4주후측정24h뇨단백、내생기항청제솔、족세포상관분자nephrin급족세포분비적VEGF단백재신장조직적표체。결과교고람총조감고제량조24h뇨단백명현저우모형조[（77.04±16.63）mg비（110.56±46.88）mg，P<0.05]，내생기항청제솔고우모형조[（1.47±0.24）mL·min-1비（1.25±0.23）mL· min-1，P<0.05]。고제량조nephrin단백표체수평교모형조명현상조（0.4250±0.02비0.3388±0.02，P<0.01），VEGF표체명현억제（0.2588±0.03비0.3521±0.01，P<0.01），차효과유사힐사탄조。결론교고람총조감능강저DN대서뇨단백，개선신공능화족세포상관단백적이상표체。
Objective To investigate the effect of gypenoside on the expressions of nephrin, vascular endothelial growth factor(VEGF) in rats with diabetic nephropathy(DN) and to explore its mechanism underlying the reduction of proteinuria and renal protection. Methods DN model was established by uninephrectomized and induced with streptozotocin, then randomly divided into model group and treatment groups (groups of high, medium, low dose of gypenoside, and valsartan group), with normal rats as control group, 8 rats in each group. After the treatment for 4 weeks, 24-hour proteinuria and clearance of creatinine(Ccr) were determined and the expression of nephrin, VEGF protein in renal tissues was examined by immunohistochemistry. Results Compared with model group, the level of 24-hour proteinuria decreased (77.04 ±16.63mg vs 110.56 ±46.88mg,P<0.05), the Ccr increased (1.47 ±0.24mg vs 1.25±0.23mg,P<0.05), the expression of nephrin protein was upregulated(0.4250±0.02 vs 0.3388±0.22,P<0.01),and the expression of VEGF was significantly reduced (0.2588±0.03 vs 0.3521±0.01,P<0.01) in high-gypenoside group. The results of high-gypenoside group were similar to valsartan group. Conclusion Gypenoside can reduce protein-uria, improve the renal function, and recover the expression of podocyteˊs associated proteins.
