浙江中西医结合杂志
浙江中西醫結閤雜誌
절강중서의결합잡지
ZHEJIANG JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2015年
1期
8-11,106
,共5页
大鼠%脊髓损伤%补阳还五汤%细胞凋亡%VEGF
大鼠%脊髓損傷%補暘還五湯%細胞凋亡%VEGF
대서%척수손상%보양환오탕%세포조망%VEGF
rats%spinal cord injury%Buyang Huanwu Decoction%Apoptosis%VEGF
目的：观察补阳还五汤对大鼠急性脊髓损伤（ASCI）的治疗作用及其机制。方法 SD大鼠随机分为空白组、损伤对照组、补阳还五汤组，各10只。采用Allen’s打击法建立急性脊髓损伤模型，空白组和损伤对照组造模后予蒸馏水1mL灌胃，补阳还五汤组造模后予补阳还五汤1mL灌胃，均1天1次，连续3天。各组分别于术后1、7、14天观察其行为学BBB评分，14天通过免疫组化的方法观察脊髓损伤部位神经细胞凋亡（caspase-3p20、Bcl-2与Bax）、血管内皮生长因子（VEGF）的表达水平。结果①行为学观察评分：术后7、14天补阳还五汤组与损伤对照组下肢功能均有恢复（P<0.05），且补阳还五汤组较损伤对照组恢复更明显[（9.05±0.95）分比（5.75±1.97）分，（12.6±1.55）分比（8.80±3.34）分，P<0.05]。②免疫组化检测：补阳还五汤组caspsase-3p20阳性细胞数表达较损伤对照组少[（16.70±2.21）个比（20.30±4.13）个，P<0.05]；补阳还五汤组Bcl-2阳性细胞的表达较损伤对照组增加[（10.57±0.97）个比（6.42±3.20）个，P<0.05]；补阳还五汤组Bax阳性细胞的表达较损伤对照组少[（18.29±2.21）个比（21.14±2.41）个，P<0.05]；补阳还五汤组VEGF阳性细胞的表达较损伤对照组多[（11.75±1.03）个比（9.50±1.30）个，P<0.05]。结论补阳还五汤可提高脊髓损伤部位VEGF的表达，使细胞凋亡明显减少，促进骨髓功能的恢复。
目的：觀察補暘還五湯對大鼠急性脊髓損傷（ASCI）的治療作用及其機製。方法 SD大鼠隨機分為空白組、損傷對照組、補暘還五湯組，各10隻。採用Allen’s打擊法建立急性脊髓損傷模型，空白組和損傷對照組造模後予蒸餾水1mL灌胃，補暘還五湯組造模後予補暘還五湯1mL灌胃，均1天1次，連續3天。各組分彆于術後1、7、14天觀察其行為學BBB評分，14天通過免疫組化的方法觀察脊髓損傷部位神經細胞凋亡（caspase-3p20、Bcl-2與Bax）、血管內皮生長因子（VEGF）的錶達水平。結果①行為學觀察評分：術後7、14天補暘還五湯組與損傷對照組下肢功能均有恢複（P<0.05），且補暘還五湯組較損傷對照組恢複更明顯[（9.05±0.95）分比（5.75±1.97）分，（12.6±1.55）分比（8.80±3.34）分，P<0.05]。②免疫組化檢測：補暘還五湯組caspsase-3p20暘性細胞數錶達較損傷對照組少[（16.70±2.21）箇比（20.30±4.13）箇，P<0.05]；補暘還五湯組Bcl-2暘性細胞的錶達較損傷對照組增加[（10.57±0.97）箇比（6.42±3.20）箇，P<0.05]；補暘還五湯組Bax暘性細胞的錶達較損傷對照組少[（18.29±2.21）箇比（21.14±2.41）箇，P<0.05]；補暘還五湯組VEGF暘性細胞的錶達較損傷對照組多[（11.75±1.03）箇比（9.50±1.30）箇，P<0.05]。結論補暘還五湯可提高脊髓損傷部位VEGF的錶達，使細胞凋亡明顯減少，促進骨髓功能的恢複。
목적：관찰보양환오탕대대서급성척수손상（ASCI）적치료작용급기궤제。방법 SD대서수궤분위공백조、손상대조조、보양환오탕조，각10지。채용Allen’s타격법건립급성척수손상모형，공백조화손상대조조조모후여증류수1mL관위，보양환오탕조조모후여보양환오탕1mL관위，균1천1차，련속3천。각조분별우술후1、7、14천관찰기행위학BBB평분，14천통과면역조화적방법관찰척수손상부위신경세포조망（caspase-3p20、Bcl-2여Bax）、혈관내피생장인자（VEGF）적표체수평。결과①행위학관찰평분：술후7、14천보양환오탕조여손상대조조하지공능균유회복（P<0.05），차보양환오탕조교손상대조조회복경명현[（9.05±0.95）분비（5.75±1.97）분，（12.6±1.55）분비（8.80±3.34）분，P<0.05]。②면역조화검측：보양환오탕조caspsase-3p20양성세포수표체교손상대조조소[（16.70±2.21）개비（20.30±4.13）개，P<0.05]；보양환오탕조Bcl-2양성세포적표체교손상대조조증가[（10.57±0.97）개비（6.42±3.20）개，P<0.05]；보양환오탕조Bax양성세포적표체교손상대조조소[（18.29±2.21）개비（21.14±2.41）개，P<0.05]；보양환오탕조VEGF양성세포적표체교손상대조조다[（11.75±1.03）개비（9.50±1.30）개，P<0.05]。결론보양환오탕가제고척수손상부위VEGF적표체，사세포조망명현감소，촉진골수공능적회복。
Objective To determine the therapeutic efficacy of Buyang Huanwu Decoction (BYHWD) in the treatment of acute spinal cord injury(ASCI) in rats, and to investigate the underlying mechanism. Methods Thirty SD rats were randomized into control group(laminectomy only), ASCI group, ASCI+BYHWD group, 10 rats in each group. ASCI was induced by using the modified weight-drop method in the latter two groups. Rats in control and ASCI groups were intragastrically given distilled water once daily for 3d,rats in ASCI+BYHWD group were given BYHWD for the same period. Locomotors functional recovery was assessed on day 1,7,14 after operation by per-forming open-field locomotors tests(BBB score). On postoperative day 14,the segments of spinal cord encompassing the injury site were removed for histopathological analysis. Immunohistochemistry was performed to observe the ex-pression of B-cell CLL/lymphoma-2(Bcl-2),BCL-2-associated X protein(Bax),caspase-3,and vascular endothelial growth factor(VEGF). Results Both of the BYHWD-treated rats and ASCI rats had locomotor function recovery on postoperative day 7 and 14(P<0.05),but the BYHWD-treated rats had significantly better locomotor function recov-ery than ASCI group did (D7:9.05±0.95 vs 5.75±1.97;D14:12.60±1.55 vs 8.80±3.34;all P<0.05). Compared with ASCI group,the BYHWD-treated rats had reduced expression of caspase-3(16.70±2.21 vs 20.30±4.13,P<0.05) and Bax(18.29±2.21 vs 21.14±2.41,P<0.05),increased expression of Bcl-2(10.57±0.97 vs 6.42±3.20,P<0.05) and VEGF (11.75±1.03 vs 9.50±1.30,P<0.05). Conclusion BYHWD treatment after ASCI can significantly improve locomotor recovery by upregulating VEGF and inhibiting neural apoptosis.
