CAJ | 학술논문

目的：观察电针对脑缺血再灌注模型大鼠血清白介素-6(interleukin-6，IL-6)、IL-8和IL-10的影响，探讨电针防治缺血性脑病的作用机理。方法：雄性Sprague Dawley (SD)大鼠按随机数字表分为假手术组、模型组和电针组，以上三组又各分为6 h和24 h两个亚组。假手术组仅予手术血管分离，插入线栓，不进行治疗。模型组及电针组采用改良Longa线栓法复制大脑中动脉局灶性脑缺血再灌注模型。模型组仅造模，不作任何治疗；电针组治疗取百会(GV 20)及大椎(GV 14)，采用电针疏密波刺激30 min。应用Elisa 法测定各组大鼠IL-6、IL-8和IL-10水平。结果：脑缺血再灌注后6 h，模型组血清IL-6、IL-8及IL-10水平均较假手术组高(P＜0.01, P＜0.05, P＜0.05)；电针组血清IL-8水平低于模型组(P＜0.05)，血清IL-6及IL-10水平与模型组无统计学差异。脑缺血再灌注后24 h，电针组血清IL-6及IL-8水平低于模型组(均P＜0.05)，血清IL-10水平三组比较均无统计学差异。结论：电针早期介入脑缺血损伤可以调节血清IL-6、IL-8水平。
목적：관찰전침대뇌결혈재관주모형대서혈청백개소-6(interleukin-6，IL-6)、IL-8화IL-10적영향，탐토전침방치결혈성뇌병적작용궤리。방법：웅성Sprague Dawley (SD)대서안수궤수자표분위가수술조、모형조화전침조，이상삼조우각분위6 h화24 h량개아조。가수술조부여수술혈관분리，삽입선전，불진행치료。모형조급전침조채용개량Longa선전법복제대뇌중동맥국조성뇌결혈재관주모형。모형조부조모，불작임하치료；전침조치료취백회(GV 20)급대추(GV 14)，채용전침소밀파자격30 min。응용Elisa 법측정각조대서IL-6、IL-8화IL-10수평。결과：뇌결혈재관주후6 h，모형조혈청IL-6、IL-8급IL-10수평균교가수술조고(P＜0.01, P＜0.05, P＜0.05)；전침조혈청IL-8수평저우모형조(P＜0.05)，혈청IL-6급IL-10수평여모형조무통계학차이。뇌결혈재관주후24 h，전침조혈청IL-6급IL-8수평저우모형조(균P＜0.05)，혈청IL-10수평삼조비교균무통계학차이。결론：전침조기개입뇌결혈손상가이조절혈청IL-6、IL-8수평。
Objective: To observe the effect of electroacupuncture (EA) on serum interleukin (IL)-6, IL-8 and IL-10 in rat models of cerebral ischemia-reperfusion, and to discover the mechanism of EA in preventing and treating cerebral ischemia. <br> Methods:Male Sprague Dawley (SD) rats were randomized into a sham-operation (SO) group, a model control (MC) group, and an EA group, which were sub-grouped into a 6-hour group and a 24-hour group. In the SO group, rats only received vessel separation with filament placed inside without any treatment. In the MC and EA groups, the focal cerebral ischemia-reperfusion model was induced by using modified Longa method with intraluminal filament. The MC group didn’t receive any treatment;the EA group received EA at Baihui (GV 20) and Dazhui (GV 14) with sparse-dense wave for 30 min. The levels of serum IL-6, IL-8 and IL-10 were detected by using Elisa test. <br> Results: Six hours after ischemia-reperfusion injury, the levels of serum IL-6, IL-8 and IL-10 in the MC group were significantly higher than those in the SO group (P<0.01, P<0.05, P<0.05);the level of serum IL-8 in the EA group was significantly lower than that in the MC group (P<0.05), while there were no significant differences in comparing IL-6 and IL-10 between the EA group and the MC group. Twenty-four hours after ischemia-reperfusion injury, the levels of serum IL-6 and IL-8 in the EA group were significantly lower than those in the MC group (both P<0.05), while there were no significant differences in comparing the level of IL-10 among the three groups. <br> Conclusion:Early intervention by EA can regulate the levels of serum IL-6 and IL-8 in cerebral ischemic injury.