世界最新医学信息文摘（连续型电子期刊）
世界最新醫學信息文摘（連續型電子期刊）
세계최신의학신식문적（련속형전자기간）
World Latest Medicine Information
2014年
35期
8-8,10
,共2页
cd44+%口腔鳞癌干细胞%Wnt/β-catenin%eMt
cd44+%口腔鱗癌榦細胞%Wnt/β-catenin%eMt
cd44+%구강린암간세포%Wnt/β-catenin%eMt
cd44+%oral squamous cell carcinoma stem cells%Wnt/beta-catenin%eMt
目的：分选口腔鳞癌干细胞并进一步研究Wnt/β-catenin信号通路在鳞癌干细胞的迁移、侵袭及eMt转化中的作用。方法磁珠分选口腔鳞癌干细胞并用细胞流式技术检测其纯度；免疫印迹检测slug、twist及oct-4的表达变化；细胞划痕及transwell小室检测口腔鳞癌干细胞的迁移及侵袭能力。结果细胞流式技术检测磁珠分选得到的cd44+细胞，结果表明分选纯度达93%；细胞划痕及transwell小室检测Wnt/β-catenin信号通路在口腔鳞癌干细胞中的作用，实验数据表明激活Wnt/β-catenin途径能够显著促进肿瘤干细胞的迁移及侵袭。免疫印迹检测发现wnt3a能够明显增强口腔鳞癌干细胞中slug、twist及oct-4的表达，同时用dKK1进行抑制实验，结果显示slug、twist及oct-4的表达降低。结论 Wnt/β-catenin信号通路在cd44+的口腔鳞癌干细胞的迁移、侵袭及eMt转化中发挥重要作用，对于肿瘤的治疗有显著的指导意义。
目的：分選口腔鱗癌榦細胞併進一步研究Wnt/β-catenin信號通路在鱗癌榦細胞的遷移、侵襲及eMt轉化中的作用。方法磁珠分選口腔鱗癌榦細胞併用細胞流式技術檢測其純度；免疫印跡檢測slug、twist及oct-4的錶達變化；細胞劃痕及transwell小室檢測口腔鱗癌榦細胞的遷移及侵襲能力。結果細胞流式技術檢測磁珠分選得到的cd44+細胞，結果錶明分選純度達93%；細胞劃痕及transwell小室檢測Wnt/β-catenin信號通路在口腔鱗癌榦細胞中的作用，實驗數據錶明激活Wnt/β-catenin途徑能夠顯著促進腫瘤榦細胞的遷移及侵襲。免疫印跡檢測髮現wnt3a能夠明顯增彊口腔鱗癌榦細胞中slug、twist及oct-4的錶達，同時用dKK1進行抑製實驗，結果顯示slug、twist及oct-4的錶達降低。結論 Wnt/β-catenin信號通路在cd44+的口腔鱗癌榦細胞的遷移、侵襲及eMt轉化中髮揮重要作用，對于腫瘤的治療有顯著的指導意義。
목적：분선구강린암간세포병진일보연구Wnt/β-catenin신호통로재린암간세포적천이、침습급eMt전화중적작용。방법자주분선구강린암간세포병용세포류식기술검측기순도；면역인적검측slug、twist급oct-4적표체변화；세포화흔급transwell소실검측구강린암간세포적천이급침습능력。결과세포류식기술검측자주분선득도적cd44+세포，결과표명분선순도체93%；세포화흔급transwell소실검측Wnt/β-catenin신호통로재구강린암간세포중적작용，실험수거표명격활Wnt/β-catenin도경능구현저촉진종류간세포적천이급침습。면역인적검측발현wnt3a능구명현증강구강린암간세포중slug、twist급oct-4적표체，동시용dKK1진행억제실험，결과현시slug、twist급oct-4적표체강저。결론 Wnt/β-catenin신호통로재cd44+적구강린암간세포적천이、침습급eMt전화중발휘중요작용，대우종류적치료유현저적지도의의。
objective to sorting of oral squamous cell carcinoma stem cells and further research of Wnt/-catenin signaling pathway in squamous cell carcinoma invasion and migration of stem, and the role of eMt transformed cells. Magnetic beads separation Method of oral squamous cell carcinoma stem cells and detect the purity of the cell lfow cytometry;the expression change of slug, twist and Western blot detection of oct-4 and transwell assay;cell scratch migration and invasion ability of oral squamous cell carcinoma stem cells.Results cell flow detection magnetic bead sorting type technology of cd44+cells, results show that sorting purity reached 93%; cell scratch and transwell assay of Wnt/ -catenin signaling pathway in oral squamous cell carcinoma stem cells and the role, the experimental data show that the activation of Wnt/ beta -catenin pathway can significantly promote the migration and invasion of tumor stem cells. immunoblot assay showed that Wnt3a could signiifcantly enhance the expression of Slug in oral squamous cell carcinoma stem, Twist and OCT-4 cells, while DKK1 inhibition, showed decreased expression of slug, twist and oct-4.Conclusion Wnt/beta-catenin signaling pathway play an important role in invasion and migration of eMt cells, and the transformation of cd44+in oral squamous cell carcinoma stem, have signiifcant guiding signiifcance for tumor treatment.
