临床医药文献电子杂志
臨床醫藥文獻電子雜誌
림상의약문헌전자잡지
Journal of Clinical Medical Literature (ElectronicEdition)
2014年
12期
2178-2180
,共3页
杨春会%田新%殷海斌%高晓慧%李娜
楊春會%田新%慇海斌%高曉慧%李娜
양춘회%전신%은해빈%고효혜%리나
超高液相色谱法%甲氨蝶呤%5-甲基四氢叶酸%血药浓度%急性淋巴细胞白血病
超高液相色譜法%甲氨蝶呤%5-甲基四氫葉痠%血藥濃度%急性淋巴細胞白血病
초고액상색보법%갑안접령%5-갑기사경협산%혈약농도%급성림파세포백혈병
High performance liquid chromatography (HPLC) method%Methotrexate%5-methyl 4 hydrogen folic acid%Blood drug concentration%Acute lymphoblastic leukemia
目的：研究旨在探讨超高液相色谱（Ultrahigh pressure liquid chromatography， UPLC）法检测的精准性及5-甲基四氢叶酸（5-methyltetrahydrofolate，5-MTHF）测定方法学的建立。方法用UPLC法检测不同间隔时间及浓度MTX、CF及5-MTHF样品，分析UPLC法检测的精准性。结果 UPLC法检测3种浓度（1.0 mg/L 4.0 mg/L 16.0 mg/L）的血浆样品室温放置48h后测定的浓度与标示量的相对偏差分别为MTX 5.6%、5%、2.5%；CF 2%、5%、5.6%；5-MCHF 6.0%、7.5%、5.6%；-80摄氏度冻融1月后测定的浓度与标示量的相对偏差分别为MTX 5.6%、5%、2.5%；CF1.2%、2.5%、1.3%；5-MCHF5.4%、2.5%、6.3%。结论本法测定儿童血浆中5-MTHF的浓度时结果稳定，重现性好，可以用于临床大剂量MTX化疗后CF解救的患儿体内血浆中解救药物CF浓度监测，增加了MTX应用的进一步监测指标，为以后探讨5-MTHF浓度高时ALL患儿的复发风险提供了有力的检测手段。
目的：研究旨在探討超高液相色譜（Ultrahigh pressure liquid chromatography， UPLC）法檢測的精準性及5-甲基四氫葉痠（5-methyltetrahydrofolate，5-MTHF）測定方法學的建立。方法用UPLC法檢測不同間隔時間及濃度MTX、CF及5-MTHF樣品，分析UPLC法檢測的精準性。結果 UPLC法檢測3種濃度（1.0 mg/L 4.0 mg/L 16.0 mg/L）的血漿樣品室溫放置48h後測定的濃度與標示量的相對偏差分彆為MTX 5.6%、5%、2.5%；CF 2%、5%、5.6%；5-MCHF 6.0%、7.5%、5.6%；-80攝氏度凍融1月後測定的濃度與標示量的相對偏差分彆為MTX 5.6%、5%、2.5%；CF1.2%、2.5%、1.3%；5-MCHF5.4%、2.5%、6.3%。結論本法測定兒童血漿中5-MTHF的濃度時結果穩定，重現性好，可以用于臨床大劑量MTX化療後CF解救的患兒體內血漿中解救藥物CF濃度鑑測，增加瞭MTX應用的進一步鑑測指標，為以後探討5-MTHF濃度高時ALL患兒的複髮風險提供瞭有力的檢測手段。
목적：연구지재탐토초고액상색보（Ultrahigh pressure liquid chromatography， UPLC）법검측적정준성급5-갑기사경협산（5-methyltetrahydrofolate，5-MTHF）측정방법학적건립。방법용UPLC법검측불동간격시간급농도MTX、CF급5-MTHF양품，분석UPLC법검측적정준성。결과 UPLC법검측3충농도（1.0 mg/L 4.0 mg/L 16.0 mg/L）적혈장양품실온방치48h후측정적농도여표시량적상대편차분별위MTX 5.6%、5%、2.5%；CF 2%、5%、5.6%；5-MCHF 6.0%、7.5%、5.6%；-80섭씨도동융1월후측정적농도여표시량적상대편차분별위MTX 5.6%、5%、2.5%；CF1.2%、2.5%、1.3%；5-MCHF5.4%、2.5%、6.3%。결론본법측정인동혈장중5-MTHF적농도시결과은정，중현성호，가이용우림상대제량MTX화료후CF해구적환인체내혈장중해구약물CF농도감측，증가료MTX응용적진일보감측지표，위이후탐토5-MTHF농도고시ALL환인적복발풍험제공료유력적검측수단。
Objective The study was to explore ultra high performance liquid chromatography (Ultrahigh pressure liquid chromatography, UPLC) method to detect precision and folinic acid (5 - methyl (5 -methyltetrahydrofolate, 5 - MTHF) determination of the establishment of the methodology. Methods Using UPLC method to detect different interval time and the concentration of MTX, CF and samples of 5 -MTHF analysis UPLC method detection precision. Results The UPLC method to detect three kinds of concentration (1.0 mg/L, 4.0 mg/L, 16.0 mg/L) of plasma samples at room temperature for 48 h after the determination of the concentration and the relative deviation of the amount of MTX 5.6%, 5%and 5.6%respectively;CF were 2%, 5%, 5.6%; 5 - MCHF 6.0%, 7.5%, 5.6%; - 80 degrees Celsius freeze-thaw in January after the determination of the concentration and the relative deviation of the amount of MTX were 5.6%, 5% and 5.6%respectively; CF were 1.2%, 2.5%, 1.3%; 5 - MCHF 5.4%, 2.5% and 6.3%.Conclusion The determination of 5-MTHF in plasma of children this method when the results are stable, provides a powerful means for the detection of recurrence risk after the discussion of the higher concentration of 5-MTHF in children with ALL.
