CAJ | 학술논문

本试验旨在研究分析市场上正在广泛使用的饲料添加剂，探讨二次饲喂雄性幼犬后饲料添加剂蓄积对其生长、生殖发育的影响研究。选用12只1月龄犬，随机分为试验组和对照组，对照组犬饲喂正常日粮和大鼠鼠肉，试验组犬饲喂正常日粮和高剂量组大鼠鼠肉，喂养幼犬直至成熟，检测食用饲料添加剂高剂量组大鼠鼠肉是否对雄性犬的生长、生殖发育产生影响。采用酶联免疫试剂盒的方法来检测犬血清激素指标水平。结果显示：试验组雌二醇、促卵泡激素含量明显升高（P＜0.01），而睾酮含量明显降低，有显著性差异（P＜0.01），饲料添加剂高剂量组对犬血清中的促黄体生成未见显著变化。雄性幼犬食用试验大鼠后可导致雄性幼犬青春期体内内分泌激素含量异常，即青春期11月龄测定的雌二醇以及促卵泡激素水平增高，睾酮的水平降低。提示试验组雄性幼犬食用大鼠鼠肉后，可能通过干扰了睾丸轴的功能，对雄性幼犬的生殖发育能力产生影响。
본시험지재연구분석시장상정재엄범사용적사료첨가제，탐토이차사위웅성유견후사료첨가제축적대기생장、생식발육적영향연구。선용12지1월령견，수궤분위시험조화대조조，대조조견사위정상일량화대서서육，시험조견사위정상일량화고제량조대서서육，위양유견직지성숙，검측식용사료첨가제고제량조대서서육시부대웅성견적생장、생식발육산생영향。채용매련면역시제합적방법래검측견혈청격소지표수평。결과현시：시험조자이순、촉란포격소함량명현승고（P＜0.01），이고동함량명현강저，유현저성차이（P＜0.01），사료첨가제고제량조대견혈청중적촉황체생성미견현저변화。웅성유견식용시험대서후가도치웅성유견청춘기체내내분비격소함량이상，즉청춘기11월령측정적자이순이급촉란포격소수평증고，고동적수평강저。제시시험조웅성유견식용대서서육후，가능통과간우료고환축적공능，대웅성유견적생식발육능력산생영향。
This experiment aims to study and analysis the feed additives widely used on the mar-ket, and discuss the effect of feed additives accumulation on growth and reproductive development of the second-feed male pups. 12 one-month-old male pups were select to randomly divided into ex-perimental and control groups. The control group was fed the normal diet and rat meat. The experi-mental group was fed the normal diet and high-dose rat meat. After the dogs mature, to test if high-dose rat meat could effect on male pups growth and reproductive development. Detected indica-tors of dogs serum hormone levels by using ELISA kits method. The results showed that: in experi-mental group the estradiol and follicle-stimulating hormone levels were significantly increased (P<0.01), testosterone levels significantly decreased (P<0.01), serm luteinizing was no signifi-cant change in high-dose group. Feeding rats can lead male pups endocrine hormones levels to be abnormal: 11-month-old male pups, estradiol and follicle stimulating hormone levels increased, testosterone level reduced. There was an impact on the reproductive development of male pups through interfering with the function of testicles in tip experimental groups of male pups fed rat meat.