CAJ | 학술논문

【目的】分离发病兔场皮肤真菌病的病原，并对其ITS序列进行分析鉴定。【方法】采集病兔患病部位的皮屑、结痂进行培养，并根据形态学的特点进行鉴定；然后采用真菌ITS区通用引物对其ITS区进行PCR扩增测序，并将其ITS区序列与GenBank中的核酸数据库进行比对分析，确定病原真菌的生物学分类，最后对其亲缘关系进行系统发育分析。【结果】分离得到的病原真菌经形态学初步鉴定为小孢子菌属；序列比对和系统发育分析表明，该菌与Microsporum gypseum strain WCH-MG001（序列号：GU348990.1）的ITS序列具有99%的同源性，在系统发育树上也属于同一分支，从而确定该菌为石膏样小孢子菌。
【목적】분리발병토장피부진균병적병원，병대기ITS서렬진행분석감정。【방법】채집병토환병부위적피설、결가진행배양，병근거형태학적특점진행감정；연후채용진균ITS구통용인물대기ITS구진행PCR확증측서，병장기ITS구서렬여GenBank중적핵산수거고진행비대분석，학정병원진균적생물학분류，최후대기친연관계진행계통발육분석。【결과】분리득도적병원진균경형태학초보감정위소포자균속；서렬비대화계통발육분석표명，해균여Microsporum gypseum strain WCH-MG001（서렬호：GU348990.1）적ITS서렬구유99%적동원성，재계통발육수상야속우동일분지，종이학정해균위석고양소포자균。
A bst r act: [Objective] Rabbit Dermatophytes were isolated and their ITS sequences were analyzed and identified. [Method] The collected scurf and scab were cultured,and then identified by morphological traits;the fungal ITS region-specific primers were used to perform amplification and sequencing of the ITS. Through the blast analysis between ITS sequences and GenBank nucleic acid database,the biological classifications were identified and finally their relationships were analyzed according to the phylogenetic tree. [Result] The isolated rabbit Dermatophytes were preliminary identified as Microsporum by morphological characteristics;the results of sequence analysis and phylogenetic tree showed that the isolated fungi and Microsporum gypseum strain WCH-MG001(Accession Number:GU348990.1) belonged to one group with 99%homology,indicating that the isolated fungi were Microsporum gypseum.