CAJ | 학술논문

以大米与小麦粒作为主要材料,设计4组培养基栽培双梭孢虫草(Cordyceps bifusispora),比较子实体干重。采用高效液相色谱法测定子实体中虫草菌素和腺苷含量,采用3,5-二硝基水杨酸(DNS)法测定多糖含量。结果表明：在室内自然条件下,依据子实体干重,双梭孢虫草较适宜的栽培培养基为小麦粒加营养液1(20 g葡萄糖,5 g蛋白胨,3 g KH2PO4,1.5 g MgSO4,5 mg VB1)；子实体的虫草菌素含量(103.1±15.7~122.1±18.6μg/g)各实验组间无显著性差异；依据子实体腺苷含量,适宜栽培培养基为小麦粒加营养液2(10 g葡萄糖,10 g酵母膏,1 g CaCl2,0.5 g MgSO4,0.5 g ZnSO4,0.1 g VB1),为1337.5±75.2μg/g；依据子实体多糖含量,适宜栽培培养基为大米加营养液2,为251.1±0.7 mg/g。
이대미여소맥립작위주요재료,설계4조배양기재배쌍사포충초(Cordyceps bifusispora),비교자실체간중。채용고효액상색보법측정자실체중충초균소화선감함량,채용3,5-이초기수양산(DNS)법측정다당함량。결과표명：재실내자연조건하,의거자실체간중,쌍사포충초교괄의적재배배양기위소맥립가영양액1(20 g포도당,5 g단백동,3 g KH2PO4,1.5 g MgSO4,5 mg VB1)；자실체적충초균소함량(103.1±15.7~122.1±18.6μg/g)각실험조간무현저성차이；의거자실체선감함량,괄의재배배양기위소맥립가영양액2(10 g포도당,10 g효모고,1 g CaCl2,0.5 g MgSO4,0.5 g ZnSO4,0.1 g VB1),위1337.5±75.2μg/g；의거자실체다당함량,괄의재배배양기위대미가영양액2,위251.1±0.7 mg/g。
Fruit body yields, together with cordycepin, adenosine and polysaccharide contents, were determined following cultivation of Cordyceps bifusispora on four different media containing either rice or wheat as the major component.Highest fruit body yields were recorded following cultivation on a medium consisting of 30 g wheat and 40 mL of a nutrient solution containing (per litre)20 g glucose,5 g peptone,3 g KH2 PO4 ,1.5 g MgSO4 and 5 mg vitamin B1 .No significant differences in the cordycepin levels (103.1 ± 15.7-122.1 ± 18.6 μg/g)were observed in fruit bodies cultivated on all four media,whereas highest adenosine levels (1337.5±75.2μg/g)were recorded following cultivation on a medium consisting of 30 g wheat and 40 mL of a nutrient solution containing (per litre)10 g glucose,10 g yeast extract,1 g CaCl2 ,0.5 g MgSO4,0.5 g ZnSO4and 0.1 g vitamin B1.Highest polysaccharide levels (251.1±0.7 mg/g)were recorded in fruit bodies grown on a medium consisting of 30 g rice and 40 mL of a nutrient solution containing (per litre)10 g glucose,10 g yeast extract,1 g CaCl2 ,0.5 g MgSO4 ,0.5 g ZnSO4 and 0.1 g vitamin B1 .