海洋科学
海洋科學
해양과학
MARINE SCIENCES
2014年
12期
40-47
,共8页
曾霖%雷霁霖%刘滨%洪万树%艾春香
曾霖%雷霽霖%劉濱%洪萬樹%艾春香
증림%뢰제림%류빈%홍만수%애춘향
大菱鲆(Scophthal musmaximus)幼鱼%盐度%氨基酸%脂肪酸
大蔆鲆(Scophthal musmaximus)幼魚%鹽度%氨基痠%脂肪痠
대릉평(Scophthal musmaximus)유어%염도%안기산%지방산
Scophthalmus maximus%juveniles%salinity%amino acid%fatty acid
为了探讨盐度对幼鱼肌肉中氨基酸和脂肪酸组成的影响,作者将平均体质量为(7.16±0.07) g 的大菱鲆(Scophthal musmaximus)幼鱼分别饲养在盐度(12、18、24、30(对照组)和36)水体中60 d。结果表明,不同盐度下,幼鱼肌肉中的氨基酸总量(TAA)、必需氨基酸(EAA)总量、非必需氨基(NEAA)总量和呈味氨基酸(FAA)总量分别在69.25%±0.37%~74.85%±1.26%、31.70%±0.11%~33.74%±0.28%、31.29%±0.44%~34.60%±1.26%和24.20%±0.66%~26.98%±1.02%,从高到低依次为36组>30组>12组>18组>24组,36组均显著高于24组(P <0.05)。支链氨基酸(BCAA)总量、EAA/TAA、EAA/NEAA、FAA/TAA 分别在14.94%±0.34%~15.37%±0.18%、44.57%±1.20%~45.78%±0.26%、80.40%±3.93%~84.42%±0.89%和34.95%±0.70%~36.41%±1.33%,各盐度组间均无显著性差异(P>0.05)。必需氨基酸指数(EAAI)在40.41±1.23~44.46±0.54,30和36组显著大于其他组(P<0.05)。脂肪酸(TFA)总量和非必需脂肪酸(NEFA)总量分别在88.65%±0.68%~89.66%±0.32%和85.11%±0.78%~86.38%±0.37%,12和30组均显著高于18组(P<0.05)。多不饱和脂肪酸(PUFAS)总量和 EPA+DHA 总量分别在34.95%±1.01%~36.88%±0.64%和23.53%±1.30%~25.74%±0.25%,从高到低依次为36组>30组>24组>12组>18组,各盐度组间均无显著性差异(P>0.05)。必需脂肪酸(EFA)总量在3.28%±0.04%~3.58%±0.10%,各盐度组间均无显著性差异(P>0.05)。饱和脂肪酸(SFA)总量在27.51%±0.37%~29.83%±0.35%,12组显著高于36组(P<0.05)。与对照组相比,36组大菱鲆幼鱼肌肉中的氨基酸和脂肪酸组成有所改善,其蛋白质和脂肪质量得到提高。研究成果可为大菱鲆渗透压调节的能量代谢机制及肌肉营养品质的环境调节机理积累基础资料。
為瞭探討鹽度對幼魚肌肉中氨基痠和脂肪痠組成的影響,作者將平均體質量為(7.16±0.07) g 的大蔆鲆(Scophthal musmaximus)幼魚分彆飼養在鹽度(12、18、24、30(對照組)和36)水體中60 d。結果錶明,不同鹽度下,幼魚肌肉中的氨基痠總量(TAA)、必需氨基痠(EAA)總量、非必需氨基(NEAA)總量和呈味氨基痠(FAA)總量分彆在69.25%±0.37%~74.85%±1.26%、31.70%±0.11%~33.74%±0.28%、31.29%±0.44%~34.60%±1.26%和24.20%±0.66%~26.98%±1.02%,從高到低依次為36組>30組>12組>18組>24組,36組均顯著高于24組(P <0.05)。支鏈氨基痠(BCAA)總量、EAA/TAA、EAA/NEAA、FAA/TAA 分彆在14.94%±0.34%~15.37%±0.18%、44.57%±1.20%~45.78%±0.26%、80.40%±3.93%~84.42%±0.89%和34.95%±0.70%~36.41%±1.33%,各鹽度組間均無顯著性差異(P>0.05)。必需氨基痠指數(EAAI)在40.41±1.23~44.46±0.54,30和36組顯著大于其他組(P<0.05)。脂肪痠(TFA)總量和非必需脂肪痠(NEFA)總量分彆在88.65%±0.68%~89.66%±0.32%和85.11%±0.78%~86.38%±0.37%,12和30組均顯著高于18組(P<0.05)。多不飽和脂肪痠(PUFAS)總量和 EPA+DHA 總量分彆在34.95%±1.01%~36.88%±0.64%和23.53%±1.30%~25.74%±0.25%,從高到低依次為36組>30組>24組>12組>18組,各鹽度組間均無顯著性差異(P>0.05)。必需脂肪痠(EFA)總量在3.28%±0.04%~3.58%±0.10%,各鹽度組間均無顯著性差異(P>0.05)。飽和脂肪痠(SFA)總量在27.51%±0.37%~29.83%±0.35%,12組顯著高于36組(P<0.05)。與對照組相比,36組大蔆鲆幼魚肌肉中的氨基痠和脂肪痠組成有所改善,其蛋白質和脂肪質量得到提高。研究成果可為大蔆鲆滲透壓調節的能量代謝機製及肌肉營養品質的環境調節機理積纍基礎資料。
위료탐토염도대유어기육중안기산화지방산조성적영향,작자장평균체질량위(7.16±0.07) g 적대릉평(Scophthal musmaximus)유어분별사양재염도(12、18、24、30(대조조)화36)수체중60 d。결과표명,불동염도하,유어기육중적안기산총량(TAA)、필수안기산(EAA)총량、비필수안기(NEAA)총량화정미안기산(FAA)총량분별재69.25%±0.37%~74.85%±1.26%、31.70%±0.11%~33.74%±0.28%、31.29%±0.44%~34.60%±1.26%화24.20%±0.66%~26.98%±1.02%,종고도저의차위36조>30조>12조>18조>24조,36조균현저고우24조(P <0.05)。지련안기산(BCAA)총량、EAA/TAA、EAA/NEAA、FAA/TAA 분별재14.94%±0.34%~15.37%±0.18%、44.57%±1.20%~45.78%±0.26%、80.40%±3.93%~84.42%±0.89%화34.95%±0.70%~36.41%±1.33%,각염도조간균무현저성차이(P>0.05)。필수안기산지수(EAAI)재40.41±1.23~44.46±0.54,30화36조현저대우기타조(P<0.05)。지방산(TFA)총량화비필수지방산(NEFA)총량분별재88.65%±0.68%~89.66%±0.32%화85.11%±0.78%~86.38%±0.37%,12화30조균현저고우18조(P<0.05)。다불포화지방산(PUFAS)총량화 EPA+DHA 총량분별재34.95%±1.01%~36.88%±0.64%화23.53%±1.30%~25.74%±0.25%,종고도저의차위36조>30조>24조>12조>18조,각염도조간균무현저성차이(P>0.05)。필수지방산(EFA)총량재3.28%±0.04%~3.58%±0.10%,각염도조간균무현저성차이(P>0.05)。포화지방산(SFA)총량재27.51%±0.37%~29.83%±0.35%,12조현저고우36조(P<0.05)。여대조조상비,36조대릉평유어기육중적안기산화지방산조성유소개선,기단백질화지방질량득도제고。연구성과가위대릉평삼투압조절적능량대사궤제급기육영양품질적배경조절궤리적루기출자료。
The amino acid and fatty acid compositions in muscles of juvenile turbot (Scophthalmus maximus) (body weight of 7.16g±0.07g) were investigated, which have been reared at salinities 12, 18, 24, 30 and 36 for 60 days. The results showed that total amino acid (TAA), essential amino acids (EAA), nonessential amino acids (NEAA) and flavor amino acids (FAA) contents in muscles of juvenile turbot were 69.25%±0.37%~74.85%±1.26%, 31.70%±0.11%~33.74%±0.28%, 31.29%±0.44%~34.60%±1.26% and 24.20%±0.66%~26.98%±1.02%, respectively. The content gradient was showed as follows:salinity 36>salinity 30>salinity 12>salinity 18>salinity 24, and those of fish reared at salinity 36 were signifi-cantly higher than those of fish maintained at salinity 24 (P<0.05). The contents of branched chain amino acids (BCAA), EAA/TAA, EAA/NEAA, FAA/TAA were 14.94%±0.34%~15.37%±0.18%, 44.57%±1.20%~45.78%±0.26%, 80.40%±3.93%~84.42%±0.89%and 34.95%±0.70%~36.41%± 1.33%, respectively. There were no significant differences between groups (P>0.05). The essential amino acid index (EAAI) was 40.41±1.23~44.46±0.54, increasing with the rise of water salinity. The EAAI of fish reared at salinities 30 and 36 was significant higher than those of other salinity groups (P<0.05). The total fatty acids (TFA) and non-essential fatty acids (NEFA) contents were 88.65%±0.68%~89.66%±0.32%and 85.11%±0.78%~86.38%± 0.37%, respectively. The TFA and NEFA of fish reared at salinities 12 and 30 were significant higher than those of fish adapted to salinity 18 (P<0.05). The contents of polyunsaturated fatty acids (PUFAS) and EPA+DHA were 34.95%±1.01%~36.88%±0.64%and 23.53%±1.30%~25.74%±0.25%, respectively. The content gradient was showed as follows:salinity 36>salinity 30>salinity 24>salinity 12>salinity 18, all of which had no significant dif-ferences between groups (P>0.05). The essential fatty acids (EFA) content was 3.28%±0.04%~3.58%±0.10%, no significant differences were observed between groups (P>0.05). The saturated fatty acids (SFA) content was 27.51%± 0.37%~29.83%±0.35%. The EFA in fish maintained at salinity 12 was significant higher than that of fish reared at salinity 36 (P<0.05). Therefore, the qualities of free amino acid and fatty acid in muscles of fish reared at salinity 36 had been greatly improved in comparison with those of the control (salinity 30). This study provides important references for mecha-nistic researches on energy metabolisms of osmoregulation and salinity selection in turbot mariculture.
