海洋科学
海洋科學
해양과학
MARINE SCIENCES
2014年
12期
29-33
,共5页
冯大伟%姜鹏%李富超%秦松%赵瑾%陈华新
馮大偉%薑鵬%李富超%秦鬆%趙瑾%陳華新
풍대위%강붕%리부초%진송%조근%진화신
浒苔%糖化工艺%酸水解%酶水解
滸苔%糖化工藝%痠水解%酶水解
호태%당화공예%산수해%매수해
Ulva prolifera%saccharification%acid hydrolysis%enzymatic hydrolysis
分别用质量分数为0.6%、1.0%、1.4%、1.8%、2.2%的稀硫酸在121℃高温高压的条件下水解鲜浒苔30、60、90 min,浒苔水解残留物再用纤维素酶酶解,用DNS法测定上述各步骤中的还原糖产量。结果表明,浒苔总糖含量为67.2%。稀硫酸水解工艺中,硫酸质量分数为1.8%、水解90 min,浒苔还原糖转化率最高,达59.9%,每克干质量浒苔可产生447.0 mg还原糖。稀硫酸水解残留物纤维素酶酶解工艺中,浒苔还原糖转化率较低,在硫酸质量分数为0.6%、水解时间为60 min时还原糖转化率出现最大值,仅为6.5%。结果显示浒苔稀硫酸水解产还原糖效果显著,但并不能促进纤维素酶酶解。浒苔糖化可单独采用稀硫酸水解工艺,本实验条件下的最佳工艺条件为1.8%硫酸水解90 min。本研究探索了浒苔糖化工艺条件,对后续的浒苔燃料乙醇发酵研究具有一定的参考意义。
分彆用質量分數為0.6%、1.0%、1.4%、1.8%、2.2%的稀硫痠在121℃高溫高壓的條件下水解鮮滸苔30、60、90 min,滸苔水解殘留物再用纖維素酶酶解,用DNS法測定上述各步驟中的還原糖產量。結果錶明,滸苔總糖含量為67.2%。稀硫痠水解工藝中,硫痠質量分數為1.8%、水解90 min,滸苔還原糖轉化率最高,達59.9%,每剋榦質量滸苔可產生447.0 mg還原糖。稀硫痠水解殘留物纖維素酶酶解工藝中,滸苔還原糖轉化率較低,在硫痠質量分數為0.6%、水解時間為60 min時還原糖轉化率齣現最大值,僅為6.5%。結果顯示滸苔稀硫痠水解產還原糖效果顯著,但併不能促進纖維素酶酶解。滸苔糖化可單獨採用稀硫痠水解工藝,本實驗條件下的最佳工藝條件為1.8%硫痠水解90 min。本研究探索瞭滸苔糖化工藝條件,對後續的滸苔燃料乙醇髮酵研究具有一定的參攷意義。
분별용질량분수위0.6%、1.0%、1.4%、1.8%、2.2%적희류산재121℃고온고압적조건하수해선호태30、60、90 min,호태수해잔류물재용섬유소매매해,용DNS법측정상술각보취중적환원당산량。결과표명,호태총당함량위67.2%。희류산수해공예중,류산질량분수위1.8%、수해90 min,호태환원당전화솔최고,체59.9%,매극간질량호태가산생447.0 mg환원당。희류산수해잔류물섬유소매매해공예중,호태환원당전화솔교저,재류산질량분수위0.6%、수해시간위60 min시환원당전화솔출현최대치,부위6.5%。결과현시호태희류산수해산환원당효과현저,단병불능촉진섬유소매매해。호태당화가단독채용희류산수해공예,본실험조건하적최가공예조건위1.8%류산수해90 min。본연구탐색료호태당화공예조건,대후속적호태연료을순발효연구구유일정적삼고의의。
Based on the improved two-stage sulfuric acid hydrolysis method, the total sugar content of Ulva prolif-era was determined as 67.2%. The samples of fresh U. prolifera were mixed with gradient dilute sulfuric acid (0.6、1.0、1.4、1.8、2.2%(w/w) ) and hydrolyzed at 121℃with reaction time of 30, 60 and 90 min, the solid U. prolifera residues recovered after sulfuric acid hydrolysis were hydrolyzed by cellulases, the yield of the reducing sugars from each step was determined by DNS method. It was showed that in the process of acid hydrolysis, the highest conversion rate of reducing sugars was obtained as 59.9%when the concentration of the sulfuric acid was 1.8%and the hydrolyzing time was 90 min, the yield of reducing sugars was 447.0 mg every 1000 mg dry sample. However, in the process of enzymatic hydrolysis, the conversion rate of reducing sugars from residue was low, the maxium value was only 6.5%when the concentration of sulfuric acid was 0.6%and the hydrolyzing time was 60 min. It was revealed that the acid hydrolysis was effective to hydrolyze U. prolifera sugars independently, and the optimized condition in this experiment was 1.8%(w/w) sulfuric acid with 90 min of hydrolyzing time, this will improve the further research on transferring the U. prolifera as a potential material to produce fuel ethanol.
