CAJ | 학술논문

本文总结了羊成纤维细胞建系及生物学特性检测技术，包括羊成纤维细胞原代、传代培养与纯化、冷冻保存与复苏、细胞活力测定、生长曲线、染色体分析、同工酶分析、微生物检测等，并回顾了体细胞技术已经取得的成就，展望其在我国现代畜牧业中的应用前景。
본문총결료양성섬유세포건계급생물학특성검측기술，포괄양성섬유세포원대、전대배양여순화、냉동보존여복소、세포활력측정、생장곡선、염색체분석、동공매분석、미생물검측등，병회고료체세포기술이경취득적성취，전망기재아국현대축목업중적응용전경。
This text has given a summary about goat and sheep fibroblast cell line construction technique and biological characteristics detection, including initial fibroblast cell culture, transfer of culture and purification, cell cryopreservation and resuscitation, testing cell vital force, testing cell growth curve, cell karyotype analysis, et al. The aim was to review achieve-ments of the technique of fibroblast cell line construction, and to look into the future of its us-ing in modern animal husbandry.