CAJ | 학술논문

本试验对大连某奶牛养殖场15头患临床型乳房炎病牛进行病原菌分离、鉴定。使用鉴别培养基对乳样中的大肠杆菌、葡萄球菌、链球菌及沙门氏菌进行分离，并对分离出的大肠杆菌进行血清学“O”抗原检测试验。结果显示，25个乳区的乳样共分离到病原菌37株，其中大肠杆菌15株、链球菌13株、葡萄球菌8株、沙门氏菌1株，乳房炎阳性率为5.2%，乳区阳性率为2.2%；大肠杆菌分离株以O51、O148、O78、O60血清型为主。
본시험대대련모내우양식장15두환림상형유방염병우진행병원균분리、감정。사용감별배양기대유양중적대장간균、포도구균、련구균급사문씨균진행분리，병대분리출적대장간균진행혈청학“O”항원검측시험。결과현시，25개유구적유양공분리도병원균37주，기중대장간균15주、련구균13주、포도구균8주、사문씨균1주，유방염양성솔위5.2%，유구양성솔위2.2%；대장간균분리주이O51、O148、O78、O60혈청형위주。
In this study, 15 dairy cows with bovine mastitis were tested for pathogens. Differen-tial mediums were used to isolate Escherichia coli, Staphylococcus, Streptococcus and Salmonella. Using Escherichia coli O antigen to detect the serotype. And the results showed that in 25 breast areas, 37 bacteria strains were collected. 15 of them were Escherichia coli, 13 of them were Strep-tococcus, 8 of them were Staphylococcus and 1 was Salmonella. And serotype tests showed that O51, O148, O78 and O60 were the dominant serotype in this study.