CAJ | 학술논문

弓形虫可感染多种动物引起严重的弓形虫病，为了对不同宿主感染的弓形虫株基因型差异及不同基因型弓形虫虫株的致病力差异进行进一步研究，弓形虫基因型的分析多采用限制性片段长度多态性PCR（PCR-RFLP）、随机扩增多态性DNA、多位点PCR-RFLP分析、DNA序列分析和微卫星DNA序列分析等技术，扩增位点多选择SAG1、SAG2、SAG3、BTUB和GRA6等遗传标记。传统的弓形虫基因型主要有3个，随着基因型研究的不断深入，越来越多的基因型被发现。本文就弓形虫基因分型研究进展进行讨论分析。
궁형충가감염다충동물인기엄중적궁형충병，위료대불동숙주감염적궁형충주기인형차이급불동기인형궁형충충주적치병력차이진행진일보연구，궁형충기인형적분석다채용한제성편단장도다태성PCR（PCR-RFLP）、수궤확증다태성DNA、다위점PCR-RFLP분석、DNA서렬분석화미위성DNA서렬분석등기술，확증위점다선택SAG1、SAG2、SAG3、BTUB화GRA6등유전표기。전통적궁형충기인형주요유3개，수착기인형연구적불단심입，월래월다적기인형피발현。본문취궁형충기인분형연구진전진행토론분석。
Toxoplasma gondii can cause severe toxoplasmosis infection in a variety of animals. In order to further investigate the differences in genotype of Toxoplasma gondii infection strains in different hosts and Virulence differences of different genotypes of Toxoplasma gondii strains. Toxoplasma genotype analysis by multi-use restriction fragment length polymorphism PCR(PCR-RFLP), Random amplified polymorphic DNA, multiple loci PCR-RFLP analysis, DNA sequence analysis, and the technology of microsatellite DNA sequence analysis, etc. Choose more amplification loci as SAG1, SAG2, SAG3, BTUB and GRA6 genetic markers. Traditional Toxoplasma gondii mainly has three geno-types, with the deepening of the research, more and more genotypes were found. In this paper, we will have a discussion in genetic typing of Toxoplasma.