CAJ | 학술논문

本研究首先用猪圆环病毒2型种毒攻击Balb/c小鼠，通过病毒分离，找出可使全部小鼠感染的最低病毒含量，然后用该种毒攻击免疫后的Balb/c小鼠，评价免疫保护情况。结果显示，可使小鼠全部感染的种毒最低病毒含量为106.5 TCID50/0.1 mL，当疫苗病毒含量为105.5 TCID50/0.1 mL时即可使7/10免疫小鼠得到保护，达到了疫苗的免疫效果。该研究为现有疫苗的生产工艺改进提供了重要的参考数据。
본연구수선용저원배병독2형충독공격Balb/c소서，통과병독분리，조출가사전부소서감염적최저병독함량，연후용해충독공격면역후적Balb/c소서，평개면역보호정황。결과현시，가사소서전부감염적충독최저병독함량위106.5 TCID50/0.1 mL，당역묘병독함량위105.5 TCID50/0.1 mL시즉가사7/10면역소서득도보호，체도료역묘적면역효과。해연구위현유역묘적생산공예개진제공료중요적삼고수거。
To evaluate the immune efficacy of porcine cirovirus type 2 vaccine, the viral isola-tion method was used to get the minimum content of PCV2 which can infect all the Balb/c mice. Then, mice which had been immunized by inactivated vaccine were injected with this virus, and ana-lyzed the protective effect. Results showed that the minimum content of porcine circovirus type 2 can infect all the Balb/c mice is 106.5 TCID50/0.1 mL. The vaccine can protect the seven tenths of the mice with a viral content of 105.5 TCID50/0.1 mL, and reach to the immune effect. The research could provide an important reference data for improving the production process of existing vac-cine.