CAJ | 학술논문

为建立SDS消除奇异变形杆菌质粒的方法，了解奇异变形杆菌的质粒与奇异变形杆菌耐药性表型之间的关系，采用正交设计的试验方法研究奇异变形杆菌的消除条件，并采用琼脂糖凝胶电泳的方法进行验证，筛选出质粒消除效果最佳的试验条件。采用微量稀释法进行质粒消除前后奇异变形杆菌对13种抗生素的药敏性测定。结果显示，消除效果最好的条件为消除剂SDS浓度0.2%，温度42℃，消除72 h。将该组消除后不含质粒的奇异变形杆菌与原含质粒奇异变形杆进行耐药性试验，结果表明，10株鸡源奇异变形杆菌消除质粒后，其中9株对头孢噻呋的药物敏感性由耐药变为敏感。
위건립SDS소제기이변형간균질립적방법，료해기이변형간균적질립여기이변형간균내약성표형지간적관계，채용정교설계적시험방법연구기이변형간균적소제조건，병채용경지당응효전영적방법진행험증，사선출질립소제효과최가적시험조건。채용미량희석법진행질립소제전후기이변형간균대13충항생소적약민성측정。결과현시，소제효과최호적조건위소제제SDS농도0.2%，온도42℃，소제72 h。장해조소제후불함질립적기이변형간균여원함질립기이변형간진행내약성시험，결과표명，10주계원기이변형간균소제질립후，기중9주대두포새부적약물민감성유내약변위민감。
To establish a SDS method for eliminating Proteus mirabilis plasmid, understanding the relationship between plasmid and Proteus Bacillus drug resistance phenotype. To study the elim-ination conditions of the Proteus mirabilis using experimental orthogonal method, verified the methods with agarose gel electrophoresis, screened the best experiment conditions to eliminated the plasmid . Proteus drug sensitivity to 13 antibiotics was determined by microdilution method, then plasmid were eliminated. The results showed the best effective eliminate tconditions was agent concentration of SDS 0.2%, temperature 42 ℃, lasted for 72 hours. Then drug-resistance tests were took place to detect the susceptibility of Proteus with and without plasma. The results showed that, 10 drug-resistance strains of chicken source Proteus when eliminated plasmid, 9 strains were sensitive to ceftiofur.