CAJ | 학술논문

为研究从碎米花杜鹃叶中分离得到的化合物原花青素A-1（Proanthocyanidin A-1，简称PAA-1）的免疫增强活性，初步从细胞水平上探讨其免疫增强机制。体外试验采用流式细胞术，检测PAA-1体外对小鼠脾淋巴细胞T细胞亚群CD4和CD8单阳性和双阳性T淋巴细胞亚群百分率及CD4+/CD8+比值；体内试验通过饲喂不同剂量的PAA-1后，检测试验猪外周血淋巴细胞CD4和CD8单阳性以及CD4+/CD8+比值。结果证明，体外试验中同阴性对照组相比，PAA-1能单独或协同Con A提升具CD4+、CD8+及CD4+CD8+表型的T淋巴细胞亚群百分率，提高CD4+/CD8+的比值；体内试验中饲喂中、高剂量的PAA-1的试验猪外周血中具有CD4+、CD8+表型的T淋巴细胞的百分率及CD4+/CD8+的比值均有显著提高。说明PAA-1可通过增加辅助性T淋巴细胞和细胞毒性T淋巴细胞数量、促进T淋巴细胞的成熟以及增加CD4+/CD8+的比值发挥增强细胞免疫的作用，为其进一步开发为新型免疫增强剂提供试验依据。
위연구종쇄미화두견협중분리득도적화합물원화청소A-1（Proanthocyanidin A-1，간칭PAA-1）적면역증강활성，초보종세포수평상탐토기면역증강궤제。체외시험채용류식세포술，검측PAA-1체외대소서비림파세포T세포아군CD4화CD8단양성화쌍양성T림파세포아군백분솔급CD4+/CD8+비치；체내시험통과사위불동제량적PAA-1후，검측시험저외주혈림파세포CD4화CD8단양성이급CD4+/CD8+비치。결과증명，체외시험중동음성대조조상비，PAA-1능단독혹협동Con A제승구CD4+、CD8+급CD4+CD8+표형적T림파세포아군백분솔，제고CD4+/CD8+적비치；체내시험중사위중、고제량적PAA-1적시험저외주혈중구유CD4+、CD8+표형적T림파세포적백분솔급CD4+/CD8+적비치균유현저제고。설명PAA-1가통과증가보조성T림파세포화세포독성T림파세포수량、촉진T림파세포적성숙이급증가CD4+/CD8+적비치발휘증강세포면역적작용，위기진일보개발위신형면역증강제제공시험의거。
Proanthocyanidin A-1 (PAA-1) was isolated from Rhododendron spiciferum in ethylace-tate. To preliminary study the mechanism of immuno-enhancing activity of PAA-1 in cell level, ra-tio of two main lymphocyte T subsets (CD4+ cells and CD8+ cells) were detected by flow cytometer. The lymphocyte T came from mice in vitro and peripheral blood lymphocyte, the piglets were fed with PAA-1 .The results showed that PAA-1 can elevate the CD4+, CD8+ and CD4+CD8+ cell populations alone or with Con A and elevate the ratio of CD4+/CD8+ in vitro, and enhance CD4+, CD8+ cell popula-tions and the ratio of CD4+/CD8+ in high dose groups. PAA-1 play a role in immune regulation by in-creasing the number of T helpers, T cytotoxic/suppressors and promote lymphocyte T maturation.