CAJ | 학술논문

以家猪肌肉来源 cDNA为模板,克隆了 SOCS7的编码区 cDNA 全长并发现了三种剪接变体(命名为：SOCS7-v1、SOCS7-v2和 SOCS7-v3).家猪 SOCS7编码区 cDNA 全长为1785bp,由9个外显子组成,编码含581个氨基酸残基的蛋白质.家猪SOCS7基因的三种剪接变体分别编码含547、520、512个氨基酸残基的蛋白质.通过RT-PCR方法,本研究对家猪SOCS7基因及其剪接变体的组织表达图谱进行了分析,结果显示：SOCS7在精巢、小脑、背肌、腹肌、股肌、大脑、下丘脑、心脏、肝脏、脾脏、小肠等组织中均有表达,在精巢中表达信号最强.SOCS7-v1和 SOCS7-v2除大脑外的19个检测组织中均能检测到较强的表达信号, SOCS7-v3在除精巢外的19个组织中均有表达.以上实验结果为探究家猪 SOCS7基因的生理功能奠定了基础.
이가저기육래원 cDNA위모판,극륭료 SOCS7적편마구 cDNA 전장병발현료삼충전접변체(명명위：SOCS7-v1、SOCS7-v2화 SOCS7-v3).가저 SOCS7편마구 cDNA 전장위1785bp,유9개외현자조성,편마함581개안기산잔기적단백질.가저SOCS7기인적삼충전접변체분별편마함547、520、512개안기산잔기적단백질.통과RT-PCR방법,본연구대가저SOCS7기인급기전접변체적조직표체도보진행료분석,결과현시：SOCS7재정소、소뇌、배기、복기、고기、대뇌、하구뇌、심장、간장、비장、소장등조직중균유표체,재정소중표체신호최강.SOCS7-v1화 SOCS7-v2제대뇌외적19개검측조직중균능검측도교강적표체신호, SOCS7-v3재제정소외적19개조직중균유표체.이상실험결과위탐구가저 SOCS7기인적생리공능전정료기출.
The full-length cDNA of SOCS7 was cloned using pig muscle cDNA as a template,and identi-fied three novel transcript variants (named SOCS7-v1 ,SOCS7-v2 and SOCS7-v3 ).The full-length cD-NA of SOCS7 encodes a protein of 581 amino acids,while the three novel transcript variants (SOCS7-v1,SOCS7-v2 and SOCS7-v3)encode proteins of 547,521,512 amino acids respectively.Using RT-PCR method,we further analyzed the tissue distribution of SOCS7 and its transcript variants.The re-sults showed that:the full-length SOCS7 protein is expressed in the testis,cerebellum,back muscles, abdominal muscles,femoral muscle,brain,hypothalamus,heart,liver,spleen and small intestine. While the SOCS7-v1 and SOCS7-v2 are expressed in 1 9 tissues except cerebrum;SOCS7-v3 is also ex-pressed in 1 9 tissue examined except testis.These results lay the molecular basis for further exploration of physiological roles of SOCS7 in pigs.