CAJ | 학술논문

根据GenBank中公布的脑心肌炎病毒(Encephalomyocarditis virus EMCV)3D基因保守区段设计并合成1对引物，以提取的 EMCV 核酸为模板，分别进行荧光定量 RT-PCR扩增，优化反应体系及条件，建立脑心肌炎病毒荧光定量 RT-PCR 检测方法，并初步运用于静注人免疫球蛋白(IVIG)纳米膜过滤工艺去除 EMCV效果的验证.结果显示该方法实验内和实验间的精密度均小于5%，最低检测限为102 copies/μL；纳米膜过滤工艺可使样品中的EMCV滴度下降4Logs.
근거GenBank중공포적뇌심기염병독(Encephalomyocarditis virus EMCV)3D기인보수구단설계병합성1대인물，이제취적 EMCV 핵산위모판，분별진행형광정량 RT-PCR확증，우화반응체계급조건，건립뇌심기염병독형광정량 RT-PCR 검측방법，병초보운용우정주인면역구단백(IVIG)납미막과려공예거제 EMCV효과적험증.결과현시해방법실험내화실험간적정밀도균소우5%，최저검측한위102 copies/μL；납미막과려공예가사양품중적EMCV적도하강4Logs.
Based on the conservative region of 3D gene of Encephalomyocarditis virus(EMCV )published in GenBank,a pair of primers were Designed and synthesized.Use the extracted EMCV nucleic acid as a template for fluorescence quantitative RT-PCR amplification,optimization of reaction system and condi-tions.Establishencephalomyocarditis virus (EMCV)fluorescence quantitative RT-PCR detection meth-od,and applied to static note human immunoglobulin (IVIG)nano membrane filtration process to re-move EMCV effect validation.The results showed both of the precision of intra and inter assay was <5% in Ct,and the lowest detective limit was 102 copies/μL.Nano membrane filtration process can make the samples of EMCV drops fell 4 logs.