CAJ | 학술논문

目的：培美曲塞和β-榄香烯均可抑制肿瘤细胞的生长。文中探讨培美曲塞联合β-榄香烯对宫颈癌HeLa细胞增殖和凋亡的影响。方法体外培养宫颈癌HeLa细胞，将培美曲塞（浓度为38、76、152、228、304μg／mL）单独作用于HeLa细胞后24、48 h用MTT比色法检测细胞的增殖情况，实验分β-榄香烯组（125μg／mL ）、培美曲塞组（76μg／mL ）、联合用药组（培美曲塞76μg／mL，β-榄香烯125μg／mL）及空白对照组（0μg／mL），24 h后用MTT法检测细胞的增殖情况，用流式细胞术检测24 h细胞的凋亡率。结果 MTT法显示，培美曲塞浓度在38、76、152、228、304μg／mL梯度范围内对HeLa细胞有抑制作用，随浓度增加，抑制率增强。38、76、152、228、304μg／mL的培美曲塞作用24后，增殖抑制率分别为（7．24±3．78）％、（7．94±4．37）％、（11．10±2．86）％、（15．88±3．38）％、（16．52±2．54）％；其中38、76、152、228μg／mL培美曲塞抑制率两两比较的差异有统计学意义（P＜0．05）。联合用药组24 h抑制率[（49．95±5．76）％]高于β-榄香烯组[（24．36±5．59）％]和培美曲塞组[（10．69±1．37）％]，差异有统计学意义（P＜0．01）。结论培美曲塞与β-榄香烯联合应用可抑制HeLa细胞的增殖，协同促进HeLa细胞的凋亡。
목적：배미곡새화β-람향희균가억제종류세포적생장。문중탐토배미곡새연합β-람향희대궁경암HeLa세포증식화조망적영향。방법체외배양궁경암HeLa세포，장배미곡새（농도위38、76、152、228、304μg／mL）단독작용우HeLa세포후24、48 h용MTT비색법검측세포적증식정황，실험분β-람향희조（125μg／mL ）、배미곡새조（76μg／mL ）、연합용약조（배미곡새76μg／mL，β-람향희125μg／mL）급공백대조조（0μg／mL），24 h후용MTT법검측세포적증식정황，용류식세포술검측24 h세포적조망솔。결과 MTT법현시，배미곡새농도재38、76、152、228、304μg／mL제도범위내대HeLa세포유억제작용，수농도증가，억제솔증강。38、76、152、228、304μg／mL적배미곡새작용24후，증식억제솔분별위（7．24±3．78）％、（7．94±4．37）％、（11．10±2．86）％、（15．88±3．38）％、（16．52±2．54）％；기중38、76、152、228μg／mL배미곡새억제솔량량비교적차이유통계학의의（P＜0．05）。연합용약조24 h억제솔[（49．95±5．76）％]고우β-람향희조[（24．36±5．59）％]화배미곡새조[（10．69±1．37）％]，차이유통계학의의（P＜0．01）。결론배미곡새여β-람향희연합응용가억제HeLa세포적증식，협동촉진HeLa세포적조망。
Objective Pemetrexed and β-elemene can inhibit the growth of tumor cells .This study was to investigate the effect of pemetrexed combined with β-elemene on the proliferation and apoptosis of cervical cancer HeLa cells. Methods Cervical cancer HeLa cells were treated with pemetrexed at the concentrations of 38, 76, 152, 228, and 304μg/mL, and at 24 and 48 hours of treatment subjected to MTT for detection of their proliferation .The experiment included four groups , with the cells treated with β-elemene ( 125μg/mL) , pemetrexed ( 76 μg/mL ) , β-elemene ( 125 μg/mL ) +pemetrexed (76μg/mL), and nothing (blank control) for 24 hours, followed by determination of their proliferation and apoptosis by MTT and flow cytometry, respectively. Results Pemetrexed at 38, 76, 152 and 228μg/mL inhibited the proliferation of the HeLa cells in a concentration-dependent manner, with the inhibition rates of (7.24 ±3.78), (7.94 ±4.37), (11.10 ±2.86) and (15.88 ± 3.38)%at 24 hours, and (16.69 ±0.95), (22.54 ±1.53), (24.48 ±0.92) and (25.54 ±3.61)%at 48 hours, both with statis-tically significant differences between any two groups (P<0.05).Significant differences were also found in the proliferation rate of the same concentration of pemetrexed at the two time points (P<0.05).The combination of pemetrexed and β-elemene showed an inhibi-tion rate of (49.95 ±5.76)%at 24 hours, remarkably higher than (24.36 ±5.59)%in theβ-elemene group and (10.69 ±1.37)%in the pemetrexed group (P<0.01). Conclusion Pemetrexed combined with β-elemene can significantly inhibit the proliferation and synergistically accelerate the apoptosis of HeLa cells .