中国循环杂志
中國循環雜誌
중국순배잡지
CHINESE CIRCULATION JOURNAL
2014年
12期
1039-1043
,共5页
刘岗%郑欣馨%鲁洁%陈敬州%黄晓红
劉崗%鄭訢馨%魯潔%陳敬州%黃曉紅
류강%정흔형%로길%진경주%황효홍
表没食子儿茶素没食子酸酯%脂质代谢%长链非编码核糖核酸%生物芯片
錶沒食子兒茶素沒食子痠酯%脂質代謝%長鏈非編碼覈糖覈痠%生物芯片
표몰식자인다소몰식자산지%지질대사%장련비편마핵당핵산%생물심편
Epigallocatechingallate%Lipid metabolism%Long non-coding RNA%Biochip
目的:通过生物芯片技术观察茶多酚表没食子儿茶素没食子酸酯(EGCG)对HepG2细胞脂质代谢相关基因和长链非编码RNA(lncRNAs)表达的影响并探讨后两者可能的作用关系。<br> 方法:人HepG2细胞经EGCG(25μmol/L)处理24 h后,抽提RNA,并杂交至Human Transcriptome Array 2.0芯片进行mRNA和lncRNAs表达谱的分析。通过生物信息学进行靶基因预测建立两者的可能作用关系,并应用实时荧光定量多聚酶链反应(PCR )技术对芯片结果验证。<br> 结果:HepG2细胞经EGCG处理后表达差异的脂质代谢相关基因为27条,其中11条涉及胆固醇代谢。此外,芯片结果显示有285条lncRNAs表达上调或下调。靶基因预测发现多个脂代谢基因可能受lncRNAs顺式或反式调控,实时定量PCR验证与芯片结果一致。<br> 结论:茶多酚EGCG可直接改善脂质代谢包括胆固醇的代谢,lncRNAs可能参与了对脂质代谢基因的表达调控。
目的:通過生物芯片技術觀察茶多酚錶沒食子兒茶素沒食子痠酯(EGCG)對HepG2細胞脂質代謝相關基因和長鏈非編碼RNA(lncRNAs)錶達的影響併探討後兩者可能的作用關繫。<br> 方法:人HepG2細胞經EGCG(25μmol/L)處理24 h後,抽提RNA,併雜交至Human Transcriptome Array 2.0芯片進行mRNA和lncRNAs錶達譜的分析。通過生物信息學進行靶基因預測建立兩者的可能作用關繫,併應用實時熒光定量多聚酶鏈反應(PCR )技術對芯片結果驗證。<br> 結果:HepG2細胞經EGCG處理後錶達差異的脂質代謝相關基因為27條,其中11條涉及膽固醇代謝。此外,芯片結果顯示有285條lncRNAs錶達上調或下調。靶基因預測髮現多箇脂代謝基因可能受lncRNAs順式或反式調控,實時定量PCR驗證與芯片結果一緻。<br> 結論:茶多酚EGCG可直接改善脂質代謝包括膽固醇的代謝,lncRNAs可能參與瞭對脂質代謝基因的錶達調控。
목적:통과생물심편기술관찰다다분표몰식자인다소몰식자산지(EGCG)대HepG2세포지질대사상관기인화장련비편마RNA(lncRNAs)표체적영향병탐토후량자가능적작용관계。<br> 방법:인HepG2세포경EGCG(25μmol/L)처리24 h후,추제RNA,병잡교지Human Transcriptome Array 2.0심편진행mRNA화lncRNAs표체보적분석。통과생물신식학진행파기인예측건립량자적가능작용관계,병응용실시형광정량다취매련반응(PCR )기술대심편결과험증。<br> 결과:HepG2세포경EGCG처리후표체차이적지질대사상관기인위27조,기중11조섭급담고순대사。차외,심편결과현시유285조lncRNAs표체상조혹하조。파기인예측발현다개지대사기인가능수lncRNAs순식혹반식조공,실시정량PCR험증여심편결과일치。<br> 결론:다다분EGCG가직접개선지질대사포괄담고순적대사,lncRNAs가능삼여료대지질대사기인적표체조공。
Objective: To investigate the effects of epigallocatechingallate (EGCG) on lipid metabolism related gene and long non-coding RNA (lncRNA) expression proifle by biochip technology, and to explore the possible relationship between the two elements. <br> Methods: HepG2 cell was cultured with EGCG at 25μmol/L for 24 hours, the total RNA was extracted and hybridized into the biochip of Human Transcriptome Array 2.0 for mRNA and lncRNA expression profile analysis. Bioinformatics technology was used to establish the possible relationship between lncRNA and the predicted target genes;the data obtained from biochip microarray was conifrmed by real time RT-PCR examination. <br> Results: The microarray revealed that EGCG treated HepG2 cell expressed 27 differential lipid metabolism genes and 11 of them involved in cholesterol metabolism. In addition, there 285 lncRNA expressions were up-or down-regulated. Bioinformatics technology indicated that the predicted target genes for lipid metabolism might be cis-or trans-regulated by lncRNA;the data from real-time RT-PCR was consistent with the data from biochip microarray. <br> Conclusion: Tea polyphenols improves lipid metabolism and lncRNA might be involved in the regulation of lipid metabolism related gene.