CAJ | 학술논문

目的：通过生物芯片技术观察茶多酚表没食子儿茶素没食子酸酯（EGCG）对HepG2细胞脂质代谢相关基因和长链非编码RNA（lncRNAs）表达的影响并探讨后两者可能的作用关系。 　　方法：人HepG2细胞经EGCG（25μmol/L）处理24 h后，抽提RNA，并杂交至Human Transcriptome Array 2.0芯片进行mRNA和lncRNAs表达谱的分析。通过生物信息学进行靶基因预测建立两者的可能作用关系，并应用实时荧光定量多聚酶链反应（PCR ）技术对芯片结果验证。 　　结果：HepG2细胞经EGCG处理后表达差异的脂质代谢相关基因为27条，其中11条涉及胆固醇代谢。此外，芯片结果显示有285条lncRNAs表达上调或下调。靶基因预测发现多个脂代谢基因可能受lncRNAs顺式或反式调控，实时定量PCR验证与芯片结果一致。 　　结论：茶多酚EGCG可直接改善脂质代谢包括胆固醇的代谢，lncRNAs可能参与了对脂质代谢基因的表达调控。
목적：통과생물심편기술관찰다다분표몰식자인다소몰식자산지（EGCG）대HepG2세포지질대사상관기인화장련비편마RNA（lncRNAs）표체적영향병탐토후량자가능적작용관계。 　　방법：인HepG2세포경EGCG（25μmol/L）처리24 h후，추제RNA，병잡교지Human Transcriptome Array 2.0심편진행mRNA화lncRNAs표체보적분석。통과생물신식학진행파기인예측건립량자적가능작용관계，병응용실시형광정량다취매련반응（PCR ）기술대심편결과험증。 　　결과：HepG2세포경EGCG처리후표체차이적지질대사상관기인위27조，기중11조섭급담고순대사。차외，심편결과현시유285조lncRNAs표체상조혹하조。파기인예측발현다개지대사기인가능수lncRNAs순식혹반식조공，실시정량PCR험증여심편결과일치。 　　결론：다다분EGCG가직접개선지질대사포괄담고순적대사，lncRNAs가능삼여료대지질대사기인적표체조공。
Objective: To investigate the effects of epigallocatechingallate (EGCG) on lipid metabolism related gene and long non-coding RNA (lncRNA) expression proifle by biochip technology, and to explore the possible relationship between the two elements. Methods: HepG2 cell was cultured with EGCG at 25μmol/L for 24 hours, the total RNA was extracted and hybridized into the biochip of Human Transcriptome Array 2.0 for mRNA and lncRNA expression profile analysis. Bioinformatics technology was used to establish the possible relationship between lncRNA and the predicted target genes;the data obtained from biochip microarray was conifrmed by real time RT-PCR examination. Results: The microarray revealed that EGCG treated HepG2 cell expressed 27 differential lipid metabolism genes and 11 of them involved in cholesterol metabolism. In addition, there 285 lncRNA expressions were up-or down-regulated. Bioinformatics technology indicated that the predicted target genes for lipid metabolism might be cis-or trans-regulated by lncRNA;the data from real-time RT-PCR was consistent with the data from biochip microarray. Conclusion: Tea polyphenols improves lipid metabolism and lncRNA might be involved in the regulation of lipid metabolism related gene.