CAJ | 학술논문

建立蚊耐氯菊酯C6/36细胞，并探讨PR-XP1基因与蚊C6/36细胞对氯菊酯的耐药性之间关系。采用逐步诱导法筛选蚊耐氯菊酯C6/36细胞，观察耐药细胞形态变化、生长状态，测定生长曲线和半数致死剂量；利用荧光实时定量PCR鉴定PR-XP1基因在敏感细胞与耐药细胞中的表达差异。获得耐400μg/mL氯菊酯的C6/36细胞；实时定量PCR检测发现抗药基因PR-XP1在蚊耐氯菊酯C6/36细胞中表达量明显上升。表明构建蚊耐氯菊酯C6/36细胞是研究蚊虫氯菊酯耐药性的有效方法，初步证明PR-XP1基因表达升高可能与蚊虫对氯菊酯的耐药性具有一定的相关性。
건립문내록국지C6/36세포，병탐토PR-XP1기인여문C6/36세포대록국지적내약성지간관계。채용축보유도법사선문내록국지C6/36세포，관찰내약세포형태변화、생장상태，측정생장곡선화반수치사제량；이용형광실시정량PCR감정PR-XP1기인재민감세포여내약세포중적표체차이。획득내400μg/mL록국지적C6/36세포；실시정량PCR검측발현항약기인PR-XP1재문내록국지C6/36세포중표체량명현상승。표명구건문내록국지C6/36세포시연구문충록국지내약성적유효방법，초보증명PR-XP1기인표체승고가능여문충대록국지적내약성구유일정적상관성。
The aim of this experiment is to study the molecular mechanism of permethrin-resistance cell mosquito through es-tablishing C6/36 permethrin-resistance cell line and analyzing PR-XP1 gene expression change. Stepwise screening is used to establish C6/36 permethrin-resistance cell line. Growth curve assay and MTT method are used to observe the phenotype and growth features of C6/36 permethrin-resistance cell line. Real-time PCR is implied to analyze the PR-XP1 gene mRNA level with specific primes. The C6/36 permethrin-resistance cell line are established which is against up to 400μg/mL permethrin. The results of real-time PCR show the PR-XP1 gene significantly increases in C6/36 permethrin-resistance cell line. It shows that the established C6/36 permethrin-resistance cell line is an effective cell model to study the mechanism of permethrin- resistance in mosquito. It is preliminarily proved that the up-regulation of PR-XP1 gene may play a certain role in mosquito resistance to permethrin.