CAJ | 학술논문

目的研究聚丙烯酰胺凝胶电泳（ PAGE）条带中微量DNA片段的回收和再扩增．方法比较了4种方法溶解聚丙烯酰胺凝胶的效果和回收微量核酸的效率．结果和结论 Bioteke溶胶／结合液能获得极高的DNA片段回收率，可从仅含1.0～7.5 ng 核酸的PAGE胶中有效回收到足量的片段；再扩增的效果与回收效率呈正相关；将液态混合液与杂质分离是成功进行再扩增的关键．本研究构建的一套快速、高效的PAGE多态性条带回收及再扩增的方法为分析生物遗传多样性成因提供了新途径．
목적연구취병희선알응효전영（ PAGE）조대중미량DNA편단적회수화재확증．방법비교료4충방법용해취병희선알응효적효과화회수미량핵산적효솔．결과화결론 Bioteke용효／결합액능획득겁고적DNA편단회수솔，가종부함1.0～7.5 ng 핵산적PAGE효중유효회수도족량적편단；재확증적효과여회수효솔정정상관；장액태혼합액여잡질분리시성공진행재확증적관건．본연구구건적일투쾌속、고효적PAGE다태성조대회수급재확증적방법위분석생물유전다양성성인제공료신도경．
Objective]To optimize the methods of recovery and reamplification of trace DNA fragments from polymorphic polyacrylamide gel electrophoresis (PAGE) bands.[Method] Effects of four methods of dissolving polyacrylamide gel and recovery efficiencies of trace nucleic acids were obtained .[Result and conclusion] Dissolving/Bing buffer from Bioteke kit showed high recovery rates of DNA fragments from PAGE gels with only 1 .0-7 .5 ng nucleotide .Effects of amplification positively correlated with re-covery efficiencies .The separation of liquid mixture and impurities was the key to successful amplifica-tion.This research forms a fast , efficient DNA recycling and amplification method from polymorphism PAGE, which provides a new approach to analyze the causes of genetic diversity .