CAJ | 학술논문

目的提高马大杂种相思Acacia mangium ×A．auriculiformis良种的快速繁殖效率与推广种植力度．方法以马大杂种相思新生枝条带腋芽茎段为外植体，研究马大杂种相思组培快繁体系．结果和结论用质量分数为0.1％的升汞和体积分数为75％的乙醇分别处理当年新生枝条第3～5个腋芽茎段15 min和30 s，然后接种至MS培养基进行初代培养，芽诱导率为95.68％；最佳增殖培养基为改良MS＋6-BA 1.5 mg· L－1＋NAA 0.1 mg· L－1＋蔗糖30～40 g· L－1，35 d的有效增殖倍数为3.97；将增殖芽接入含IBA 600 mg· L－1的MS固体培养基上预培养4～8 h后转接至无激素1／2 MS培养基上，15 d即可全部生根；或将增殖芽直接接入1／2 MS＋IBA 1.0 mg · L－1＋NAA 0.5 mg· L－1＋蔗糖30 g· L－1生根培养基上，第15天时生根率为99.43％．将生根苗移栽至以黄心土为基质的营养袋内，存活率为94.67％．
목적제고마대잡충상사Acacia mangium ×A．auriculiformis량충적쾌속번식효솔여추엄충식력도．방법이마대잡충상사신생지조대액아경단위외식체，연구마대잡충상사조배쾌번체계．결과화결론용질량분수위0.1％적승홍화체적분수위75％적을순분별처리당년신생지조제3～5개액아경단15 min화30 s，연후접충지MS배양기진행초대배양，아유도솔위95.68％；최가증식배양기위개량MS＋6-BA 1.5 mg· L－1＋NAA 0.1 mg· L－1＋자당30～40 g· L－1，35 d적유효증식배수위3.97；장증식아접입함IBA 600 mg· L－1적MS고체배양기상예배양4～8 h후전접지무격소1／2 MS배양기상，15 d즉가전부생근；혹장증식아직접접입1／2 MS＋IBA 1.0 mg · L－1＋NAA 0.5 mg· L－1＋자당30 g· L－1생근배양기상，제15천시생근솔위99.43％．장생근묘이재지이황심토위기질적영양대내，존활솔위94.67％．
Objective]To improve the propagation efficiency and extend the planting of Acacia mangium × A.auriculiformis.[Method]This study was carried out to explore the techniques of in vitro propagation of A.mangium ×A.auriculiformis using stem segments with buds collected from 16-year plants as the ex-plant .[Result and conclusion]Current season stem segments carrying 3-5 axillary buds were sterilized with w=0.1%mercuric chloride and φ=75%alcoho1 for 15 min and 30 s respectively before they were inoculated onto MS medium .Buds could be induced successfully on MS with a shooting rate of 95.68%. The bud proliferation index was 3.97 after being transferred onto MS +6-BA 1.5 mg· L-1 +NAA 0.1 mg· L-1 +sucrose 30-40 g· L-1 for 35 d.The buds all rooted on the 15th day from inoculation onto 1/2 MS medium after pre-cultured on MS supplemented with IBA 600 mg· L-1 for 4-8 h.Inoculating the proliferated buds onto 1/2 MS+IBA 1.0 mg· L-1 +NAA 0.5 mg· L-1 +sucrose 30 g· L-1 also generated a high rooting rate of 99.43%.The survival rate reach 94.67% after the rooted plantlet are transplanted to the yellow soil medium .