国际生殖健康计划生育杂志
國際生殖健康計劃生育雜誌
국제생식건강계화생육잡지
Journal of International Reproductive Health/Family Planning
2015年
1期
51-52
,共2页
甲醛%绒毛膜绒毛%绒毛膜促性腺激素%细胞毒性,免疫
甲醛%絨毛膜絨毛%絨毛膜促性腺激素%細胞毒性,免疫
갑철%융모막융모%융모막촉성선격소%세포독성,면역
Formaldehyde%Chorionic villi%Chorionic gonadotropin%Cytotoxicity,immunologic
目的：研究甲醛对人绒毛膜滋养细胞活性及分泌功能的影响。方法：收集9例早孕人工流产患者的绒毛组织，原代培养绒毛膜滋养细胞，分别加入50，100，150，200μmol/L甲醛处理细胞48 h。用Cell Counting Kit-8（CCK-8）检测滋养细胞活性、放射免疫法检测人绒毛膜促性腺激素（hCG）水平。以DMEM/F12培养液为对照。结果：与对照组比较，100，150，200μmol/L甲醛染毒组的细胞活性显著抑制，差异有统计学意义（均P＜0.05）；各组之间hCG水平差异无统计学意义（P＞0.05）。结论：甲醛达到一定浓度时（≥100μmol/L）对人绒毛膜滋养细胞的细胞活性有毒性作用。
目的：研究甲醛對人絨毛膜滋養細胞活性及分泌功能的影響。方法：收集9例早孕人工流產患者的絨毛組織，原代培養絨毛膜滋養細胞，分彆加入50，100，150，200μmol/L甲醛處理細胞48 h。用Cell Counting Kit-8（CCK-8）檢測滋養細胞活性、放射免疫法檢測人絨毛膜促性腺激素（hCG）水平。以DMEM/F12培養液為對照。結果：與對照組比較，100，150，200μmol/L甲醛染毒組的細胞活性顯著抑製，差異有統計學意義（均P＜0.05）；各組之間hCG水平差異無統計學意義（P＞0.05）。結論：甲醛達到一定濃度時（≥100μmol/L）對人絨毛膜滋養細胞的細胞活性有毒性作用。
목적：연구갑철대인융모막자양세포활성급분비공능적영향。방법：수집9례조잉인공유산환자적융모조직，원대배양융모막자양세포，분별가입50，100，150，200μmol/L갑철처리세포48 h。용Cell Counting Kit-8（CCK-8）검측자양세포활성、방사면역법검측인융모막촉성선격소（hCG）수평。이DMEM/F12배양액위대조。결과：여대조조비교，100，150，200μmol/L갑철염독조적세포활성현저억제，차이유통계학의의（균P＜0.05）；각조지간hCG수평차이무통계학의의（P＞0.05）。결론：갑철체도일정농도시（≥100μmol/L）대인융모막자양세포적세포활성유독성작용。
Objective:To investigate the toxic effect of formaldehyde on the cellular viability and secretory function of human cytotrophoblasts at the first trimester stage. Methods:Human villi were obtained from nine patients undergoing abortion with the signed consent form. Cytotrophoblasts were extracted and cultured in vitro in medium, and then treated with formaldehyde (50,100,150,200 μmol/L) for 48 hours. The cellular viability of cytotrophoblasts was determined with Cell Counting Kit (CCK-8), while the level of human chorionic gonadotropin (hCG) in medium was assayed with radioimmunoassay. DMEM/F12 medium was used as the negative control. Results:The cellular viabilities of cytotrophoblasts in three treatment groups with ≥100μmol/L of formaldehyde was significantly decreased than that in the control group (P<0.05). The differences of the hCG levels among those groups were not significant (P>0.05). Conclusions:Formaldehyde at a higher dose (≥100μmol/L) may reduce the cellular viability of cytotrophoblasts.
