CAJ | 학술논문

目的：探讨β-咔啉类生物碱对体外培养的人胃癌细胞株 SGC-7901细胞的凋亡作用及 PTEN、ERK mRNA 表达的影响。方法体外培养人胃癌 SGC-7901细胞，用 CCK-8法测定不同浓度、不同时间β-咔啉类生物碱对胃癌细胞株的生长抑制率；用流式细胞技术检测细胞的凋亡；RT-PCR 法检测 PTEN、ERK mRNA 表达的变化。结果β-咔啉类生物碱体外能抑制人胃癌细胞株 SGC-7901细胞的增殖，促进了人胃癌 SGC-7901细胞凋亡。不同浓度的对β-咔啉类生物碱作用于人胃癌 SGC-7901细胞24、48 h 后，PTEN mRNA 的表达增高，ERK mRNA的表达减少，且呈剂量时间依赖性（P ＜0．05）。结论人胃癌 SGC-7901细胞对β-咔啉类生物碱具有药物敏感性，β-咔啉类生物碱能诱导体外培养的胃癌细胞凋亡，其作用机制可能与β-咔啉类生物碱诱导胃癌细胞中 PTEN-mRNA 的表达增高和 ERK mRNA 的表达减少有关。
목적：탐토β-잡람류생물감대체외배양적인위암세포주 SGC-7901세포적조망작용급 PTEN、ERK mRNA 표체적영향。방법체외배양인위암 SGC-7901세포，용 CCK-8법측정불동농도、불동시간β-잡람류생물감대위암세포주적생장억제솔；용류식세포기술검측세포적조망；RT-PCR 법검측 PTEN、ERK mRNA 표체적변화。결과β-잡람류생물감체외능억제인위암세포주 SGC-7901세포적증식，촉진료인위암 SGC-7901세포조망。불동농도적대β-잡람류생물감작용우인위암 SGC-7901세포24、48 h 후，PTEN mRNA 적표체증고，ERK mRNA적표체감소，차정제량시간의뢰성（P ＜0．05）。결론인위암 SGC-7901세포대β-잡람류생물감구유약물민감성，β-잡람류생물감능유도체외배양적위암세포조망，기작용궤제가능여β-잡람류생물감유도위암세포중 PTEN-mRNA 적표체증고화 ERK mRNA 적표체감소유관。
Objective To observe the effects of β-carboline alkaloid on apoptosis of human gastric cancer SGC-7901cells and expressions of PTEN and ERK mRNA.Methods The rate of SGC-7901 cells in vitro growth inhibition was determined by CCK-8 on different doses and times ofβ-carboline alkaloid.FCM was used to analyze cell apoptosis.The expressions of PTEN and ERK mRNA were detected by RT-PCR.Re-sults β-carboline alkaloid in vitro could inhibit the proliferation of SGC-7901 cells and promote their apop-tosis.The detections showed that the expression of the PTEN mRNA was significantly higher and the ex-pression of the ERK mRNA was significantly lower,with the dependence on time and concentration.Con-clusion β-carboline alkaloid can inhibit the growth of SGC-7901 cells and induce apoptosis;the down-reg-ulation of ERK mRNA and up-regulation of PTEN mRNA may be one of the mechanisms of the apoptosis.