国际生殖健康计划生育杂志
國際生殖健康計劃生育雜誌
국제생식건강계화생육잡지
Journal of International Reproductive Health/Family Planning
2015年
1期
72-74
,共3页
卵丘细胞%卵母细胞%聚合酶链反应%微阵列分析%基因表达
卵丘細胞%卵母細胞%聚閤酶鏈反應%微陣列分析%基因錶達
란구세포%란모세포%취합매련반응%미진렬분석%기인표체
Cumulus cells%Oocytes%Polymerase chain reaction%Microarray analysis%Gene expression
缺乏准确、客观、非侵入性的胚胎评估标准是目前体外受精-胚胎移植技术所面临的主要挑战之一。本文对卵丘细胞内基因表达与胚胎及卵子发育潜能之间的相关研究进行了总结,发现卵丘细胞内特定基因的表达与胚胎发育、成熟及发育潜能之间存在紧密的联系。近年来,通过实时定量逆转录聚合酶链反应(qRT-PCR)及微阵列技术筛选出一系列颗粒细胞中能够预测受精结局、胚胎形态学评分、妊娠结局等胚胎发育潜能的候选标记基因,这些基因主要涉及卵丘扩展、脂类代谢、细胞凋亡等过程。然而,卵母细胞及胚胎的发育受卵泡内、外多种因素的影响。欲确立一种全面、准确、非侵入性的胚胎发育潜能评估方法仍需更深入的研究。
缺乏準確、客觀、非侵入性的胚胎評估標準是目前體外受精-胚胎移植技術所麵臨的主要挑戰之一。本文對卵丘細胞內基因錶達與胚胎及卵子髮育潛能之間的相關研究進行瞭總結,髮現卵丘細胞內特定基因的錶達與胚胎髮育、成熟及髮育潛能之間存在緊密的聯繫。近年來,通過實時定量逆轉錄聚閤酶鏈反應(qRT-PCR)及微陣列技術篩選齣一繫列顆粒細胞中能夠預測受精結跼、胚胎形態學評分、妊娠結跼等胚胎髮育潛能的候選標記基因,這些基因主要涉及卵丘擴展、脂類代謝、細胞凋亡等過程。然而,卵母細胞及胚胎的髮育受卵泡內、外多種因素的影響。欲確立一種全麵、準確、非侵入性的胚胎髮育潛能評估方法仍需更深入的研究。
결핍준학、객관、비침입성적배태평고표준시목전체외수정-배태이식기술소면림적주요도전지일。본문대란구세포내기인표체여배태급란자발육잠능지간적상관연구진행료총결,발현란구세포내특정기인적표체여배태발육、성숙급발육잠능지간존재긴밀적련계。근년래,통과실시정량역전록취합매련반응(qRT-PCR)급미진렬기술사선출일계렬과립세포중능구예측수정결국、배태형태학평분、임신결국등배태발육잠능적후선표기기인,저사기인주요섭급란구확전、지류대사、세포조망등과정。연이,란모세포급배태적발육수란포내、외다충인소적영향。욕학립일충전면、준학、비침입성적배태발육잠능평고방법잉수경심입적연구。
One of the major challenges in in-vitro fertilization-embryo transfer (IVF-ET) technology is lack of an objective, accurate, noninvasive criteria for evaluating the developmental potential of embryo. We reviewed here the expressions of those genes in cumulus cells related to the developmental potential of oocyte, suggesting that there was a close relationship between the expression of some specific genes in human cumulus cells and oocyte development, maturation, and embryo developmental potential. Recently, using qRT-PCR and microarray technologies, a series of candidate genes in cumulus and granulosa cells were found to be possible markers to predict the developmental potential of embryo, such as those genes related to cumulus expansion, lipid metabolism, cell apoptosis and other aspects, which could be useful to predict fertilization outcomes, embryo morphology and pregnant outcomes. However, the development of oocyte and embryo could be affected by various factors. It is necessary to do more detailed study to develop a accurate, comprehensive and non-invasive test for evaluating the developmental potential of oocyte and embryo.