CAJ | 학술논문

目的了解广东省不同区域菜园土壤微生物碳代谢功能群落结构的特点与差异．方法采用Biolog 生态微平板方法分析广东的粤北、粤东、粤西和粤中4个不同区域菜园土壤微生物碳代谢群落结构．结果和结论4个不同区域的土壤微生物平均颜色变化率（ AWCD ）、Shannon 指数、Simpson 指数、McIntosh 指数变化趋势均不同，反映微生物活性的AWCD表现为：粤北＞粤东＞粤西＞粤中，表明不同区域菜园土壤微生物在碳源利用能力、微生物丰度等方面存在差异．4个区域土壤微生物对6类31种碳源的利用程度存在差异；主成分分析显示，4个区域土壤微生物代谢基质主成分1的贡献率为54．76％，主成分2为13．25％；主成分1载荷0．18以上的基质有22种，主成分2有15种；碳源在主成分分离中起主要贡献作用的是氨基酸类、羧酸类、酚类和碳水化合物类碳源．表明不同区域菜园土壤微生物群落碳源利用模式及代谢功能不同，即土壤微生物群落功能多样性有差异．
목적료해광동성불동구역채완토양미생물탄대사공능군락결구적특점여차이．방법채용Biolog 생태미평판방법분석엄동적월북、월동、월서화월중4개불동구역채완토양미생물탄대사군락결구．결과화결론4개불동구역적토양미생물평균안색변화솔（ AWCD ）、Shannon 지수、Simpson 지수、McIntosh 지수변화추세균불동，반영미생물활성적AWCD표현위：월북＞월동＞월서＞월중，표명불동구역채완토양미생물재탄원이용능력、미생물봉도등방면존재차이．4개구역토양미생물대6류31충탄원적이용정도존재차이；주성분분석현시，4개구역토양미생물대사기질주성분1적공헌솔위54．76％，주성분2위13．25％；주성분1재하0．18이상적기질유22충，주성분2유15충；탄원재주성분분리중기주요공헌작용적시안기산류、최산류、분류화탄수화합물류탄원．표명불동구역채완토양미생물군락탄원이용모식급대사공능불동，즉토양미생물군락공능다양성유차이．
Objective]To understand the characteristics and differences among the carbon catabolic diver-sities of vegetable garden soil microbes from the different regions of Guangdong Province .[Method]The community structures of vegetable garden soil microbes in the northern , eastern,western and central re-gions of Guangdong Province(NR,ER,WR and CR) were analyzed using the Biolog Eco-Plate Method.[Result and conclusion]There were different changing tendencies of the Average Well Color Development (AWCD), Shannon Index, Simpson Index and McIntosh Index in the four regions .The AWCD value, which directly reflected the microbial activities , was in the order of NR>ER>WR>CR, demonstrating the differences among the uses of carbon sources and abundance in vegetable garden soil microbes from the different regions.There were differences among utilization degrees of the 31 types, 6 classes of carbon sources by the vegetable garden soil microbes from the four regions .Through principal component analy-sis, it showed that the contribution rate of the PC 1 of the metabolism substrates of vegetable garden soil microbes in the four regions was 54.76%, PC2 was 13.25%, and there were 22 types of metabolism substrates in PC1 with over 0.18 of load,but only 15 types in PC2.Amino acids, carboxylic acids, phe-nols and carbohydrates were the four main carbon sources separating the two principal component factors . In conclusion , the carbon source utilization modes and metabolic functions of the microbes among the vegetable garden soils from the four regions differ from each other , that is, there are different functional diversities of microbial community among the vegetable garden soils from the four regions under investiga-tion.