CAJ | 학술논문

目的了解上海市动物源食品中山夫登堡沙门菌Salmonella Senftenberg的流行情况、耐药性和分子分型情况．方法于2008—2012年在上海分离鉴定得到15株动物食品源山夫登堡沙门菌，通过琼脂稀释法进行最小抑菌浓度测定，并应用脉冲场凝胶电泳技术（ PFGE）对菌株进行分子分型研究．结果和结论15株山夫登堡沙门菌有7株对磺胺异恶唑具有耐药性（46．7％），除1株对链霉素耐药外（6．67％），对其他抗生素均较为敏感．PFGE分型分为10个基因型．部分不同来源的基因型（X3， X4， X5）具有高度的相似性（88．2％），表明这些不同来源的菌株可能存在流行相关性．
목적료해상해시동물원식품중산부등보사문균Salmonella Senftenberg적류행정황、내약성화분자분형정황．방법우2008—2012년재상해분리감정득도15주동물식품원산부등보사문균，통과경지희석법진행최소억균농도측정，병응용맥충장응효전영기술（ PFGE）대균주진행분자분형연구．결과화결론15주산부등보사문균유7주대광알이악서구유내약성（46．7％），제1주대련매소내약외（6．67％），대기타항생소균교위민감．PFGE분형분위10개기인형．부분불동래원적기인형（X3， X4， X5）구유고도적상사성（88．2％），표명저사불동래원적균주가능존재류행상관성．
[Objective]To investigate the antimicrobial resistance and molecular types of Salmonella Sen-ftenberg isolates from food of animal origins in Shanghai .[Method]A total of 15 Salmonella Senftenberg i-solates were collected from 2008 to 2012 in Shanghai .Agar dilution method and pulse field gel electrophore-sis (PFGE) were applied to the determination of minimum inhibitory concentrations (MICs) and molecular typing, respectively .[Result and conclusion]The 15 isolates exhibited resistance most often to sulfisox-azole(46.7%) and they were fully sensitive to the remaining antibiotics expect for one which was resistant to streptomycin (6.67%) .PFGE analyses of 15 Salmonella Senftenberg isolates resulted in 10 unique pat-terns, among which three (X3, X4, X5) were grouped together at a high similarity index of 88.2%, su-ggesting that these isolates of different origins may be relevant in the epidemiology .